1887

Abstract

Summary

A computerised typing method based on biochemical fingerprinting was used to investigate biochemical phenotypes (BPTs) among 70 strains of of serotype Havana isolated from human cases of gastroenteritis in Iran and other parts of the world. A total of 16 BPTs comprising five common and 11 single phenotypes was identified. The most frequently found BPT contained 24 isolates from Iran and nine from other countries. Three common BPTs with two, seven and 15 isolates were found among Iranian strains only and one common BPT with two isolates was found among non-Iranian strains only. Antibiotic-resistance patterns and virulence properties of strains from these common BPTs suggested that they might be unique clones. Forty-two Iranian isolates shared multi-resistance to between three and seven antibiotics. In contrast, none of the isolates from other countries was resistant to antibiotics. Furthermore, 43 Iranian isolates showed mannose-resistant adhesion to HeLa cells and 24 of them possessed an aerobactin-mediated iron-uptake system, whereas none of the isolates from other countries possessed any of these virulence properties. These findings suggest that four unique clones of Havana with different BPTs and virulence properties are common in Iran; two particular clones were responsible for a majority of Havana infections there. However, the most prevalent BPT found among Iranian strains was also common in strains from other countries. It is concluded that biochemical fingerprinting, as used in this study, is a reliable method for identifying clonal groups of Havana strains. The method is reproducible, easy to perform and can be used alone, or in combination with other typing methods, in epidemiological studies of serotype Havana.

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1992-06-01
2023-01-31
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References

  1. Corbelo A. Martinez-Cruz., J. A. Historia de la Salmonella havana. Arch de Med Infect 1941; 10:170–179
    [Google Scholar]
  2. Pavia A. T., Shipman L. D., Wells J. G. Epidemiologic evidence,that prior antimicrobial exposure decreases resistance to infection by antimicrobial-sensitive Salmonella. J Infect Dis 1990; 161:255–260
    [Google Scholar]
  3. Fule R. P., Saoji A. M. Isolation of Salmonella havana (13, 23: f.g:-) a rare serotype from human source. Indian J Pathol Microbiol 1988; 31:67–68
    [Google Scholar]
  4. Soerjadi-Liem A. S., Cumming R. B. Studies on the incidence of Salmonella carriers in broiler hocks entering a poultry processing plant in Australia. Poultry Science 1984; 63:892–895
    [Google Scholar]
  5. Bensink J. C. Salmonella contamination of meat and bone meal. Aust Vet J 1979; 55:13–15
    [Google Scholar]
  6. Yoshimura H., Nakamura H., Sato S. Incidence of salmonellae in animal feed ingredients in Japan. Natl Inst Anim Health Q 1979; 19:107–113
    [Google Scholar]
  7. Berchieri A., Irino K., Neme S. N. Contaminacao por Salmonella em farinhas de origem animal utilizados no preparo de racao. Pesquisa Veter inaria Brasileira 1984; 4:83–88
    [Google Scholar]
  8. Farhoudi-Moghaddam A. A., Katouli M., Jafari A., Bahavar M. A., Parsi M., Malekzadeh F. Antimicrobial drug resistance and resistance factor transfer among clinical isolates of salmonellae in Iran. Scand J Infect Dis 1990; 22:197–203
    [Google Scholar]
  9. Anderson E. S., Ward L. R., de Saxe M. J., de Sa J. D. H. Bacterio phage-typing designations of Salmonella typhimurium. J Hyg 1977; 78:297–300
    [Google Scholar]
  10. Duguid J. P., Anderson E. S., Alfredsson G. A., Barker R., Old D. C. A new biotyping scheme for Salmonella typhimurium and its phylogenetic significance. J Med Microbiol 1975; 8149–166
    [Google Scholar]
  11. Barker R. M., Keaemey G. M., Nicholson P., Blair A. L., Porter R. C., Crichton P. B. Types of Salmonella paratyphi B and their phylogenetic significance. J Med Microbiol 1988; 26:285–293
    [Google Scholar]
  12. Brunner F., Margadant A., Peduzzi R., Piffaretti J. -C. The plasmid pattern as an epidemiologic tool for Salmonella typhimurium epidemics: comparison with the lysotype. J Infect Dis 1983; 148:7–11
    [Google Scholar]
  13. Threlfall E. J., Frost J. A. The identification, typing and finger printing of Salmonella: laboratory aspects and epidemiological applications. J Appl Bacteriol 1990; 68:5–16
    [Google Scholar]
  14. Mitchell E., O’Mahoney M., Lynch D. Large outbreak of food poisoning caused by Salmonella typhimurium definitive type 49 in mayonnaise. Br Med J 1989; 298:99–101
    [Google Scholar]
  15. Riley L. W., DiFerdinando G. T., DeMelfi T. M., Cohen M. L. Evaluation of isolated cases of salmonellosis by plasmid profile analysis: introduction andlransmission of bacterial clone by precooked roast beef. J Infect Dis 1983; 148:12–17
    [Google Scholar]
  16. Taylor D. N., Wachsmuth I. K., Shangkuan Y. -H. Salmonel losis associated with marijuana. A multistate outbreak traced by plasmid fingerprinting. N Engl J Med 1982; 306:1249–1253
    [Google Scholar]
  17. Kuhn I. Biochemical fingerprinting of Escherichia coli: a simple method for epidemiological investigations. J Microbiol Methods 1985; 3:159–170
    [Google Scholar]
  18. Kuhn I., Burman L. G., Eriksson L., Möllby R. Subtyping of Klebsiella by biochemical fingerprinting: a simple system for epidemiological investigations. J Microbiol Methods 1990; 11:177–185
    [Google Scholar]
  19. Bauer A.W., Kirby W. M. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45:493–96
    [Google Scholar]
  20. Sneath P. H. A., Sokal R. R. Numerical taxonomy San Francisco, CA: WH Freeman; 1973
    [Google Scholar]
  21. Cravioto A., Gross R. J., Scotland S. M., Rowe B. Adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Curr Microbiol 1979; 3:95–99
    [Google Scholar]
  22. Scaletsky I. C. A., Silva M. L. M., Trabulsi L. R. Distinctive pattern of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45:534–536
    [Google Scholar]
  23. Roberts R. B., Abelson P. H., Cowe D. B., Bolton E.T., Britten R. J. Studies of biosynthesis in Escherichia coli. Publication 607 Carnegie Institute of Washington DC; 1955
    [Google Scholar]
  24. McDonough P. L., Timoney J. F., Jacobson R. H., Khakhria R. Clonal groups of Salmonella typhimurium in New York State. J Clin Microbiol 1989; 27:622–627
    [Google Scholar]
  25. Holmberg S. D., Wachsmuth I. K., Hickman-Brenner F. W., Cohen M. L. Comparison of plasmid profile analysis, phage typing, and antimicrobial susceptibility testing in characterizing Salmonella typhimurium isolates from outbreaks. J Clin Microbiol 1984; 19:100–104
    [Google Scholar]
  26. Anderson E. S. The modern ecological study of Salmonella typhimurium infection. In Perez-Miravete A. Peleaz (eds) Recent advances in microbiology Proceedings of the Xth International Congress for Microbiology; Mexico City: 1970381
    [Google Scholar]
  27. Crichton P. B., Old D. C. A biotyping scheme for the subspecific discrimination of Escherichia coli. J Med Microbiol 1982; 15:233–242
    [Google Scholar]
  28. Simoons-Smit A. M., Verwei-van jVught A. M. J. J., Kanis I. Y. R., MacLaren D. M. Biochemical and serological investigations on clinical isolates of Klebsiella. J Hyg 1985; 95:265–276
    [Google Scholar]
  29. Eisenstein B. I. New molecular techniques for microbial epi demiology and the diagnosis of infectious diseases. J Infect Dis 1990; 161:595–602
    [Google Scholar]
  30. Katouli M., Kuhn I., Möllby R. Evaluation of the stability of biochemical phenotypes of Escherichia coli upon subculturing and storage. J Gen Microbiol 1990; 136:1681–1688
    [Google Scholar]
  31. Barker R. M., Old D. C. The usefulness of biotyping in studying the epidemiology and phylogeny of salmonellae. J Med Microbiol 1989; 29:81–88
    [Google Scholar]
  32. Barker R., Old D. C., Sharp J. C. M. Phage type/biotype groups of Salmonella typhimurium in Scotland 1974–6: variation during spread of epidemic clones. J Hyg 1980; 84:115–125
    [Google Scholar]
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