Variation of the O antigen of Free

Abstract

Summary

During the course of a clinical study on patients with campylobacteriosis, three consecutive isolates of from the same patient were sent for O-serotyping. Marked differences in the specificities of the O antigens of the isolates were observed between the first and third isolates when a passive haemagglutination assay system developed for serotyping was used. Differences in specificity were also demonstrated by immunoblots of lipopolysaccharides (LPS) from proteinase K-digested whole cells. The three isolates could not be distinguished either by restriction endonuclease analysis of chromosomal DNA, by gel electrophoresis of whole-cell proteins or by silver-stained LPS gels, thus providing evidence that they were of the same strain and that antigenic variation had occurred .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-36-3-215
1992-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/36/3/medmicro-36-3-215.html?itemId=/content/journal/jmm/10.1099/00222615-36-3-215&mimeType=html&fmt=ahah

References

  1. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subspjejuni on the basis of heat-stable antigens. J Clin Microbiol 1980; 12:732–737
    [Google Scholar]
  2. Penner J. L., Congi R. V., Hennessey J. N. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol 1983; 2:378–383
    [Google Scholar]
  3. Preston M. A., Penner J. L. Characterization of cross-reacting serotypes of Campylobacter jejuni. Can J Microbiol 1989; 35:265–273
    [Google Scholar]
  4. Bradbury W. C., Pearson A. D., Marko M. A., Congi R. V., Penner J. L. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol 1984; 19:342–346
    [Google Scholar]
  5. Melby K., Storvold G., Congi R. V., Penner J. L. Serotyping of Campylobacter jejuni isolated from sporadic cases and outbreaks in northern Norway. Acta Pathol Microbiol Scand Sect B 1985; 93:83–86
    [Google Scholar]
  6. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 1983; 154:269–277
    [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  8. Tsai C.-M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115–119
    [Google Scholar]
  9. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
  10. Simor A. E., Shames B., Drumm B., Sherman P., Low D. E., Penner J. L. Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile. J Clin Microbiol 1990; 28:83–86
    [Google Scholar]
  11. Kauffmann F. The bacteriology of Enterobacteriaceae. Copenhagen, Munksgaard: 196676–80
    [Google Scholar]
  12. Kauffmann F., Rohde R. Neue befunde beim O-Formen Wechsel der Salmonella-species. Acta Pathol Scand 1961; 52:211–216
    [Google Scholar]
  13. Mills S. D., Kurjanczyk L. A., Shames B., Hennessy J. N., Penner J. L. Antigenic shifts in serotype determinants of Campylobacter coli are accompanied by chromosomal DNA restriction endonuclease digestion pattern. J Med Microbiol 1991; 35:168–173
    [Google Scholar]
  14. Guerry P., Logan S., Trust T. J. Genomic rearrangements associated with antigenic variation in Campylobacter coli. J Bacteriol 1988; 170:316–319
    [Google Scholar]
  15. Pei Z., Ellison R. T., Lewis R. V., Blaser M. J. Purification and characterization of a family of high molecular weight surface-array proteins from Campylobacter fetus. J Biol Chem 1988; 263:6416–6420
    [Google Scholar]
  16. Wang E., Pei Z., Blaser M. J. Annual Meeting of the American Society for Microbiology. Abstract no B233:1990–125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-36-3-215
Loading
/content/journal/jmm/10.1099/00222615-36-3-215
Loading

Data & Media loading...

Most cited Most Cited RSS feed