During the course of a clinical study on patients with campylobacteriosis, three consecutive isolates of Campylobacter jejuni from the same patient were sent for O-serotyping. Marked differences in the specificities of the O antigens of the isolates were observed between the first and third isolates when a passive haemagglutination assay system developed for serotyping C. jejuni was used. Differences in specificity were also demonstrated by immunoblots of lipopolysaccharides (LPS) from proteinase K-digested whole cells. The three isolates could not be distinguished either by restriction endonuclease analysis of chromosomal DNA, by gel electrophoresis of whole-cell proteins or by silver-stained LPS gels, thus providing evidence that they were of the same strain and that antigenic variation had occurred in vivo.
PennerJ. L.,
HennessyJ. N. Passive hemagglutination technique for serotyping Campylobacter fetus subspjejuni on the basis of heat-stable antigens. J Clin Microbiol1980; 12:732–737
PennerJ. L.,
CongiR. V.,
HennesseyJ. N. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol1983; 2:378–383
BradburyW. C.,
PearsonA. D.,
MarkoM. A.,
CongiR. V.,
PennerJ. L. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol1984; 19:342–346
TowbinH.,
StaehelinT.,
GordonJ. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA1979; 76:4350–4354
SimorA. E.,
ShamesB.,
DrummB.,
ShermanP.,
LowD. E.,
PennerJ. L. Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile. J Clin Microbiol1990; 28:83–86
MillsS. D.,
KurjanczykL. A.,
ShamesB.,
HennessyJ. N.,
PennerJ. L. Antigenic shifts in serotype determinants of Campylobacter coli are accompanied by chromosomal DNA restriction endonuclease digestion pattern. J Med Microbiol1991; 35:168–173
PeiZ.,
EllisonR. T.,
LewisR. V.,
BlaserM. J. Purification and characterization of a family of high molecular weight surface-array proteins from Campylobacter fetus. J Biol Chem1988; 263:6416–6420