1887

Abstract

Summary

DNA of was cleaved by the endonuclease RI and ligated with the bacteriophage expression vector λ gt11. After infection of the strain Y1089, the plaques of recombinant phages were screened with a antiserum (human) for fusion proteins containing borrelia antigens. A positive clone produced a hybrid protein (p200) of 200 Kda. The corresponding native borrelia protein (p97) was identified as having an M of 97 Kda. To localise protein p97 in the cell, immunoelectronmicroscopy and a Western blot of isolated flagella were used. Antibodies directed against proteins p200 and p97 recognised epitopes associated with the flagella.

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/content/journal/jmm/10.1099/00222615-36-3-209
1992-03-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-36-3-209
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