1887

Abstract

Summary

A novel iron-binding compound was identified in ethyl acetate extracts of the supernates from cultures. This compound, named azurechelin, was produced by 88% of strains isolated from the respiratory tract. Production of azurechelin was regulated by the iron concentration in the culture medium. Azurechelin enhanced the growth of in a medium containing transferrin 200 mg/L. Azurechelin released iron from transferrin in an equilibrium dialysis assay, suggesting that it could compete with transferrin for iron. Azurechelin could also stimulate iron uptake by . This siderophore appeared to have a novel structure with neither the typical characteristics of catechol nor of hydroxamate compounds.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-36-3-184
1992-03-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/36/3/medmicro-36-3-184.html?itemId=/content/journal/jmm/10.1099/00222615-36-3-184&mimeType=html&fmt=ahah

References

  1. Payne S. M., Finkelstein R. A. The critical role of iron in host-bacterial interactions. J Clin Invest 1978; 61:1428–1440
    [Google Scholar]
  2. Crosa J. H. The relationship of plasmid-mediated iron transport and bacterial virulence. Annu Rev Microbiol 1984; 38:69–89
    [Google Scholar]
  3. Neilands J. B. Microbial envelope proteins related to iron. Annu Rev Microbiol 1982; 36:285–309
    [Google Scholar]
  4. Barclay R. The role of iron in infection. Med Lab Sci 1985; 42:166–177
    [Google Scholar]
  5. Isles A., Maclusky I., Corey R. Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr 1984; 104:206–210
    [Google Scholar]
  6. Sokol P. A. Production and utilization of pyochelin by clinical isolates of Pseudomonas cepacia. J Clin Microbiol 1986; 23:560–562
    [Google Scholar]
  7. Sokol P. A., Woods D. E. Effect of pyochelin on Pseudomonas cepacia respiratory infections. Microb Pathog 1988; 5:197–205
    [Google Scholar]
  8. McKevitt A. I., Woods D. E. Characterization of Pseudomonas cepacia isolates from patients with cystic fibrosis. J Clin Microbiol 1984; 19:291–293
    [Google Scholar]
  9. Cox C. D., Graham R. Isolation of an iron-binding compound from Pseudomonas aeruginosa. J Bacteriol 1979; 137:357–364
    [Google Scholar]
  10. Cox C. D. Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. J Bacteriol 1980; 142:581–587
    [Google Scholar]
  11. Schwyn B., Neilands J. B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem 1987; 160:47–56
    [Google Scholar]
  12. Arrow L. E. Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 1937; 118:531–537
    [Google Scholar]
  13. Rioux C., Jordan D. C., Rattray J. B. M. Colorimetric determination of catechol siderophores in microbial cultures. Anal Biochem 1983; 133:163–169
    [Google Scholar]
  14. Csáky Z. On the estimation of bound hydroxylamine in biological materials. Acta Chem Scand 1948; 2:450–454
    [Google Scholar]
  15. Sriyosachati S., Cox C. D. Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. Infect Immun 1986; 52:885–891
    [Google Scholar]
  16. Simonson C., Brener D., DeVoe I. W. Expression of a high-affinity mechanism for acquisition for transferrin iron by Neisseria meningitidis. Infect Immun 1982; 36:107–113
    [Google Scholar]
  17. Meyer J.-M., Hohnadel D., Halle F. Cepabactin from Pseudomonas cepacia, a new type of siderophore. J Gen Microbiol 1989; 135:1479–1487
    [Google Scholar]
  18. Menkin V. The role of the hydrogen ion concentration and the cytology of an exudate. Glycolysis in inflammation. Some aspects of the chemistry of exudates. In Ryan E. J. (ed) Biochemical mechanisms in inflammation Springfield, IL: Charles C Thomas; 195666–103
    [Google Scholar]
  19. Neilands J. B. Methodology of siderophores. Structure and Bonding 1984; 58:1–24
    [Google Scholar]
  20. Neilands J. B. Microbial iron compounds. Annu Rev Biochem 1981; 50:715–731
    [Google Scholar]
  21. Smith M. J., Shoolery J. N., Schwyn B., Holden I., Neilands J. B. Rhizobactin, a structurally novel siderophore from Rhizobium meliloti. J Am Chem Soc 1985; 107:1739–1743
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-36-3-184
Loading
/content/journal/jmm/10.1099/00222615-36-3-184
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error