The effects of purified extracellular lipase from Staphylococcus aureus on human granulocytes were studied in vitro with a turbidimetric technique. Within the concentration range 0.6–4.4 μg/ml, lipase caused monophasic aggregation accompanied by the release of lactoferrin; the corresponding concentrations of the solvent in which it was suspended, Triton X100, had no effect. Lipase-induced aggregation did not occur in the presence of autologous plasma.
SingerC. Infections in patients with neoplastic diseases. In
GriecoM. E.
(ed) Infections in the abnormal host New York: Yorke Medical Books; 1980546–584
BraconierJ. H.,
OdebergH. Granulocyte phagocytosis and killing of virulent and avirulent serotypes of Streptococcus pneumoniae. J Lab Clin Med1982; 100:279–287
PalmbladJ.,
GyllenhammarH.,
RingertzB.,
NilssonE.,
CottellB. Leukotriene B4 triggers highly characteristic and specific functional responses in neutrophils: studies of stimulus specific mechanisms. Biochim Biophys Acta1988; 971:92–102
ThelestamM.,
MӧllbyR. Determination of toxin-induced leakage of different-size nucleotides through the plasma membrane of human diploid fibroblasts. Infect Immun1975; 11:640–648
ChristenssonB.,
FehrenbachF. J.,
HedstrӧmS. A. A new serological assay for Staphylococcus aureus infections: detection of IgG antibodies to S. aureus lipase with an enzyme-linked immunosorbent assay. J Infect Dis1985; 152:286–292
RollofJ.,
HedstrӧmS. A.,
Nilsson-EhleP. Lipolytic activity of Staphylococcus aureus strains from disseminated and localized infections. APMIS Sect B1987; 95:109–113
WoodinA. M. The leukocidin-treated leucocyte. In
DingleJ. T.
(ed) Lysosomes in biology and pathology vol 3 Amsterdam, North Holland Publishing Co: 1973395–422