Reaction of cells of different morphology index with monoclonal antibodies specific for the hyphal form Free

Abstract

Summary

Two monoclonal antibodies (MAbs), 3D9 with reported specificity for hyphae, and 3B7 with reported specificity for morphological forms of found , were tested by indirect immunofluorescence with cells that were grown in 12 different environments (four different culture media incubated at various temperatures) and whose cellular morphology was estimated in terms of morphology index (Mi). Both MAbs reacted strongly with cells with Mi> 3.0, i.e., with pseudohyphal and hyphal forms, but in Eagle’s medium at 26°C and in a modified Sabouraud’s broth medium at 30°C, some reactivity was also found with cells of lower Mi (i.e., yeast forms). Therefore, it was concluded that the hyphal phenotype and the epitopes reactive with the MAbs were co-expressed but that the epitopes could also be expressed independently of the hyphal phenotype. The results confirm the propensity of for variation of its surface antigenic composition.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-35-6-321
1991-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/35/6/medmicro-35-6-321.html?itemId=/content/journal/jmm/10.1099/00222615-35-6-321&mimeType=html&fmt=ahah

References

  1. Evans EGV, Richardson MD, Odds FC, Holland KT. Relevance of antigenicity of Candida albicans growth phases to diagnosis of systemic candidiasis. Br Med J 1973; 4:86–87
    [Google Scholar]
  2. Syverson RE, Buckley HR, Campbell CC. Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect Immun 1975; 12:1184–1188
    [Google Scholar]
  3. Poulain D, Tronchin G, Vernes A, Popeye R, Biguet J. Antigenic variations of Candida albicans in vivo and in vitro—relationships between P antigens and serotypes. Sabouraudia 1983; 21:99–112
    [Google Scholar]
  4. Smail EH, Jones JM. Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes. Infect Immun 1984; 45:74–81
    [Google Scholar]
  5. Sundstrom PM, Kenny GE. Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence. Infect Immun 1984; 43:850–855
    [Google Scholar]
  6. Anderson JM, Soil DR. Unique phenotype of opaque cells in the white-opaque transition of Candida albicans. J Bacteriol 1987; 169:5579–5588
    [Google Scholar]
  7. Quindós G, Pontϳn J, Cisterna R. Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis. Eur J Clin Microbiol 1987; 6:142–146
    [Google Scholar]
  8. Sundstrom PM, Nichols EJ, Kenny GE. Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans. Infect Immun 1987; 55:616–620
    [Google Scholar]
  9. Brawner DL, Cutler JE. Variability in expression of cell surface antigens of Candida albicans during morphogenesis. Infect Immun 1986; 51:337–343
    [Google Scholar]
  10. Fortier B, Hopwood V, Poulain D. Electric and chemical fusions for the production of monoclonal antibodies reacting with the in-vivo growth phase of Candida albicans. J Med Microbiol 1988; 27:239–245
    [Google Scholar]
  11. Brawner DL, Cutler JE, Beatty WL. Caveats in the investigation of form-specific molecules of Candida albicans. Infect Immun 1990; 58:378–383
    [Google Scholar]
  12. Merson-Davies LA, Odds FC. A morphology index for characterization of cell shape in Candida albicans. J Gen Microbiol 1989; 135:3143–3152
    [Google Scholar]
  13. Evans EGV, Odds FC, Richardson MD, Holland KT. Optimum conditions for initiation of filamentation in Candida albicans. Can J Microbiol 1975; 21:338–342
    [Google Scholar]
  14. Hopwood V, Poulain D, Fortier B, Evans G, Vernes A. A monoclonal antibody to a cell wall component of Candida albicans. Infect Immun 1986; 54:222–227
    [Google Scholar]
  15. Page S, Odds FC. Binding of plasma proteins to Candida species in vitro. J Gen Microbiol 1988; 134:2693–2702
    [Google Scholar]
  16. Annaix V, Aubry J, Robert R, Bouali A, Tronchin G, Senet J-M. Fibrinogen binding of Candida albicans germ tubes and mycelium. Protides Biol Fluids 1987; 35:399–402
    [Google Scholar]
  17. Brawner DL, Cutler JE. Cell surface and intracellular expression of two Candida albicans antigens during in vitro and in vivo growth. Microb Pathog 1987; 2:249–257
    [Google Scholar]
  18. Poulain D, Vernes A, Fruit J. Variation de la structure antigénique parietale de Candida albicans: mise en évidence au niveau des blastospores d’un antigène P dependant de leur origine. Sabouraudia 1980; 18:61–68
    [Google Scholar]
  19. Poulain D, Tronchin G, Lefebvre B, Husson MO. Antigenic variability between Candida albicans blastospores isolated from healthy subjects and patients with Candida infection. Sabouraudia 1982; 20:173–177
    [Google Scholar]
  20. Poulain D, Hopwood V, Vernes A. Antigenic variability of Candida albicans. CRC Crit Rev Microbiol 1985; 12:223–270
    [Google Scholar]
  21. Fruit J, Cailliez JC, Odds FC, Poulain D. Expression of an epitope by surface glycoproteins of Candida albicans. Variability among species, strains and yeast cells of the genus Candida. J Med Vet Mycol 1990; 28:241–252
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-35-6-321
Loading
/content/journal/jmm/10.1099/00222615-35-6-321
Loading

Data & Media loading...

Most cited Most Cited RSS feed