1887

Abstract

Summary

A collection of 143 strains of enteropathogenic (EPEC) of 11 different serogroups isolated from children with diarrhoea, 71 in Sweden and 72 in Iran, was tested for similarity with a computerised biochemical fingerprinting method. From Sweden, there were 54 different phenotypes, 42 consisting of a single strain and 12 (common phenotypes) containing more than one isolate. From Iran, there were 48 different phenotypes, 38 with only one strain and 10 with more than one. Many of the strains which were biochemically similar, in both countries, also had similar virulence factors. Nine Swedish and 20 Iranian isolates showed biochemical identity to at least one of the strains of the other country, most of them belonging to serogroups O55, O119, O126, O127 and O128. The value of the biochemical fingerprinting method as an epidemiological tool and its ability to evaluate clonal relations among strains in different geographical areas is discussed.

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1991-11-01
2024-12-08
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References

  1. Neter E. Enteritis due to enteropathogenic Escherichia coli: present-day status and undissolved problems. J Pediatr 1959; 55:223–239
    [Google Scholar]
  2. Robins-Browne RM. Traditional enteropathogenic Escherichia coli of infantile diarrhea. Rev Infect Dis 1987; 9:28–53
    [Google Scholar]
  3. Czirók É, Ørskov I, Ørskov F. O: K: H: F serotypes of fimbriated Escherichia coli strains isolated from infants with diarrhea. Infect Immun 1982; 37:519–525
    [Google Scholar]
  4. Ørskov F, Ørskov I. Summary of workshop on the clone concept in epidemiology, taxonomy, and evolution of the Enterobacteriaceae and other bacteria. J Infect Dis 1983; 148:346–357
    [Google Scholar]
  5. Stenderup J, Ørskov F. The clonal nature of enteropathogenic Escherichia coli strains. J Infect Dis 1983; 148:1019–1024
    [Google Scholar]
  6. Crichton PB, Old DC. A biotyping scheme for the subspecific discrimination of Escherichia coli. J Med Microbiol 1982; 15:233–242
    [Google Scholar]
  7. Brauner A, Boeufgras J-M, Jacobson SH. et al. The use of biochemical markers, serotypes and fimbriation in the detection of Escherichia coli clones. J Gen Microbiol 1987; 133:2825–2834
    [Google Scholar]
  8. Kühn I. Biochemical fingerprinting of Escherichia coli: a simple method for epidemiological investigations. J Microbiol Methods 1985; 3:159–170
    [Google Scholar]
  9. Kühn I, Brauner A, Möllby R. Evaluation of numerical typing systems for Escherichia coli using the API 50 CH and the PhP-EC systems as models. Epidemiol Infect 1990; 105:521–531
    [Google Scholar]
  10. Sneath PHA. Numerical taxonomy. San Francisco: W. H. Freeman; 1973
    [Google Scholar]
  11. Kühn I, Franklin A, Söderlind O, Möllby R. Phenotypic variations among enterotoxinogenic Escherichia coli from Swedish piglets with diarrhoea. Med Microbiol Immunol 1985; 174:119–130
    [Google Scholar]
  12. Scaletsky ICA, Silva MLM, Trabulsi LR. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984; 45:534–536
    [Google Scholar]
  13. Baloda SB, Yuk YR, Krovacek K, Sethi SK, Wadström T. Detection of Shiga-Like (SL) toxins of enteropathogenic Escherichia coli (EPEC) of human, porcine, calf and lamb origin on Vero and HeLa S3 cells: a comparative study. ZentralblBakteriolMikrobiolHyg A 1987; 264:33–40
    [Google Scholar]
  14. Konowalchuk J, Speirs JI, Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun 1977; 18:775–779
    [Google Scholar]
  15. Achtman M, Mercer A, Kusecek B. et al. Six widespread bacterial clones among Escherichia coli isolates. Infect Immun 1983; 39:315–335
    [Google Scholar]
  16. Ochman H, Seiander RK. Evidence for clonal population structure in Escherichia coli. Proc Natl Acad Sci USA 1984; 81:198–201
    [Google Scholar]
  17. Rennie RP, Nord CE, Sjoberg L, Duncan IBR. Comparison of bacteriophage typing, serotyping and biotyping as aids in epidemiological surveillance of Klebsiella infections. J Clin Microbiol 1978; 8:638–642
    [Google Scholar]
  18. Katouli M, Kühn I, Möllby R. Evaluation of the stability of biochemical phenotypes of Escherichia coli upon subculturing and storage. J Gen Microbiol 1990; 136:1681–1688
    [Google Scholar]
  19. Levine MM, Ristanio P, Sack RB, Kapper JB, Ørskov F, Ørskov I. Colonization factor antigens I and II and type 1 somatic pili in enterotoxigenic Escherichia coli: relation to enterotoxin type. Infect Immun 1983; 39:889–897
    [Google Scholar]
  20. Evans DJ, Evans DG, DuPont HL, Ørskov F, Ørskov I. Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect Immun 1977; 17:105–111
    [Google Scholar]
  21. Âhrén CM, Gothefors L, Stoll BJ, Salek MA, Svennerholm A-M. Comparison of methods for detection of colonization factor antigens on enterotoxigenic Escherichia coli. J Clin Microbiol 1986; 23:586–591
    [Google Scholar]
  22. Katouli M, Jaafari A, Ketabi GR. The role of diarrhoeagenic Escherichia coli in acute diarrhoeal diseases in Bandar-Abbas, Iran. J Med Microbiol 1988; 27:71–74
    [Google Scholar]
  23. Katouli M, Jaafari A, Farhoudi-Moghaddam AA, Ketabi GR. Aetiological studies of diarrhoeal diseases in infants and young children in Iran. J Trop Med Hyg 1990; 93:22–27
    [Google Scholar]
  24. Bettelheim KA. The sources of “OH” serotypes of Escherichia coli. J Hyg 1978; 80:83–113
    [Google Scholar]
  25. Ørskov F, Ørskov I, Evans DJ, Sack RB, Sack DA, Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhea in adults and children. Med Microbiol 1976; 162:73–80
    [Google Scholar]
  26. Gaugant DA, Levin BR, Ørskov I, Ørskov F, Svanborg Eden C, Seiander RK. Genetic diversity in relation to serotype in Escherichia coli. Infect Immun 1985; 49:407–413
    [Google Scholar]
  27. Kühn I, Möllby R. Phenotypic variations among enterotoxigenic O-groups of Escherichia coli from various human populations. Med Microbiol Immunol 1986; 175:15–26
    [Google Scholar]
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