RT Journal Article SR Electronic(1) A1 Wylie, B. A. A1 Koornhof, H. J.YR 1991 T1 Nucleotide sequence of dihydrofolate reductase type VI JF Journal of Medical Microbiology, VO 35 IS 4 SP 214 OP 218 DO https://doi.org/10.1099/00222615-35-4-214 PB Microbiology Society, SN 1473-5644, AB Summary The complete sequence of the type VI dihydrofolate reductase (DHFR) gene from plasmid pUK672 was determined. The structural gene coded for a polypeptide of 157 amino acids which had a deduced mol. wt of 17 424. Comparison with amino-acid sequences of the type I, type V and Escherichia coli K12 chromosomal DHFRs showed that there was 63%, 61% and 31% homology respectively. Putative RNA polymerase and ribosomal binding sites were identified proximal to the initiation codon and a feature consistent with transcription termination was present distal to the coding region. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed that the enzyme had a subunit mol. wt of 17 500., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-35-4-214