%0 Journal Article %A Saunders, N. A. %A Harrison, T. G. %A Haththotuwa, A. %A Taylor, A. G. %T A comparison of probes for restriction fragment length polymorphism (RFLP) typing of Legionella pneumophila serogroup 1 strains %D 1991 %J Journal of Medical Microbiology, %V 35 %N 3 %P 152-158 %@ 1473-5644 %R https://doi.org/10.1099/00222615-35-3-152 %I Microbiology Society, %X Summary The use of probes derived from rRNA sequences to detect restriction fragment length polymorphisms (RFLPs) associated with the ribosomal RNA genes for epidemiological typing (ribotyping) is a powerful and readily applicable tool. Different probes and enzymes for ribotyping were compared for a series of 73 unrelated Legionella pneumophila serogroup 1 strains. The probes compared were cDNAs, transcribed from L. pneumophila or Escherichia coli rRNA subunits, and a cloned L. pneumophila rRNA gene. The cloned rRNA gene probe gave the best discrimination and this probe was further compared with cloned probes comprised of randomly selected (non-rRNA) parts of the L. pneumophila chromosome. In this instance the greatest discrimination was achieved when one of the non-ribosomal RNA gene probes was employed. The overall discrimination of RFLP typing was enhanced by combining the data obtained with both rRNA and non-rRNA probes. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-35-3-152