1887

Abstract

Summary

The hypothesis was investigated that tissue tropism of during colonisation and infection is associated with the ability of fimbriate bacteria to bind to the organs and cell types involved. type b with fimbriae (strain 770235f) bound to several cell types, including ciliated columnar epithelial cells, pneumocytes, ependymal cells, glial cells, connective tissue fibroblasts, synovial cells, antigen-presenting cells, lymphocytes, erythrocytes and endothelial cells. Binding of to kidney, liver and conjunctiva cells was poor. Fimbriae-specific monoclonal antibody (MAb 6HE8) inhibited this binding. Some binding to endothelial cells and macrophages was also observed with non-fimbriate strains. This binding was not inhibited by MAb 6HE8. We conclude that in-vitro binding of fimbriate is mainly to those tissues and cells where is found during colonisation and infection. The data suggest that a shift to the nonfimbriate form is required for bacteria in the bloodstream to escape clearance mechanisms mediated by blood cells.

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1991-09-01
2022-01-22
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