1887

Abstract

. The clinical isolate WBG1024 was resistant to cadmium, benzyl penicillin, kanamycin, neomycin, streptomycin, tetracycline and trimethoprim and harboured a conjugative plasmid pWBG637 (34.5 kb) and non-conjugative plasmids of 23.8, 4.4, 2.8 and 1.9 kb. Transduction and mixed-culture transfer experiments demonstrated that the 4.4-kb plasmid (pWBG632) encoded resistance to tetracycline and the 23.8-kb plasmid (pWBG628) encoded resistance to cadmium, benzyl penicillin, kanamycin, neomycin and streptomycin. The conjugative plasmid pWBG637 was able to mobilise a further 4.4-kb plasmid (pWBG633) encoding streptomycin resistance and recombined with the multiresistance plasmid pWBG628 to produce transconjugants of various resistance phenotypes.

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1991-08-01
2024-04-20
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References

  1. Ritz HL, Baldwin JN. Induction of penicillinase production in staphylococci by bacteriophage. Bacteriol Proc 1958; 58:40
    [Google Scholar]
  2. Lacey RW. Evidence for two mechanisms of plasmid transfer in mixed cultures of Staphylococcus aureus. J Gen Microbiol 1980; 119:423–435
    [Google Scholar]
  3. Archer GL, Johnston JL. Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrob Agents Chemother 1983; 24:70–77
    [Google Scholar]
  4. Forbes BA, Schaberg DR. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. J Bacteriol 1983; 153:627–634
    [Google Scholar]
  5. McDonnell RW, Sweeney HM, Cohen S. Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother 1983; 23:151–160
    [Google Scholar]
  6. Townsend DE, Bolton S, Ashdown N, Grubb WB. Transfer of plasmid-borne aminoglycoside-resistance determinants in staphylococci. J Med Microbiol 1985; 20:169–185
    [Google Scholar]
  7. Udo E, Townsend DE, Grubb WB. A conjugative staphylococcal plasmid with no resistance phenotype. FEMS Microbiol Lett 1987; 40:279–283
    [Google Scholar]
  8. Townsend DE, Grubb WB, Ashdown N. Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology 1983; 15:169–174
    [Google Scholar]
  9. Townsend DE, Ashdown N, Greed LC, Grubb WB. Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. J Antimicrob Chemother 1984; 14:115–124
    [Google Scholar]
  10. Townsend DE, Bolton S, Ashdown N, Annear DI, Grubb WB. Conjugative, staphylococcal plasmids carrying hitch-hiking transposons similar to Tn554: intra- and interspecies dissemination of erythromycin resistance. Aust J Exp Biol Med Sci 1986; 64:367–379
    [Google Scholar]
  11. Townsend DE, Ashdown N, Bolton S, Grubb WB. The use of cetyltrimethylammonium bromide for the rapid isolation from Staphylococcus aureus of relaxable and non-relaxable plasmid DNA for in vitro manipulation. Lett Appl Microbiol 1985; 1:87–94
    [Google Scholar]
  12. Udo EE, Grubb WB. A new class of conjugative plasmid in Staphylococcus aureus. J Med Microbiol 1990; 31:207–212
    [Google Scholar]
  13. Grubb WB, Annear DI. Unstable drug resistance in Staphylo-coccus aureus M4. Genet Res 1981; 38:217–223
    [Google Scholar]
  14. Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98:503–517
    [Google Scholar]
  15. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982
    [Google Scholar]
  16. Lacey RW. Properties of an unusual genetic element in Staphylococcus aureus. J Med Microbiol 1979; 12:311–319
    [Google Scholar]
  17. Kigbo EP, Townsend DE, Ashdown N, Grubb WB. Transposition of penicillinase determinants in methicillin-resistant Staphylococcus aureus. FEMS Microbiol Lett 1985; 28:39–43
    [Google Scholar]
  18. Weber DA, Goering RV. Tn4201, a β-lactamase transposon in Staphylococcus aureus. Antimicrob Agents Chemother 1988; 32:1164–1169
    [Google Scholar]
  19. Shalita Z, Murphy E, Novick RP. Penicillinase plasmids of Staphylococcus aureus. Structural and evolutionary relationships. Plasmid 1980; 3:291–311
    [Google Scholar]
  20. Skurray RA, Rouch DA, Lyon BR. et al. Multiresistant Staphylococcus aureus: genetics and evolution of epidemic Australian strains. J Antimicrob Chemother 1988; 21:Suppl C19–38
    [Google Scholar]
  21. Projan SJ, Archer GL. Mobilisation of the relaxable Staphylo-coccus aureus plasmid pC221 by the conjugative plasmid pGOl involves three pC221 loci. J Bacteriol 1989; 171:1841–1845
    [Google Scholar]
  22. Goering RV, Asch DK. Plasmid mobilisation effected by a conjugal aminoglycoside resistance plasmid in Staphylococ-eus aureus. Zentralbl Backteriol Parasitenkd Infektionskr Hyg 1985 Abt 1 Suppl 14 563–565
    [Google Scholar]
  23. Novick RP, Projan SJ, Rosenblum W, Edelman I. Staphylococcal plasmid cointegrates are formed by host- and phage-mediated general rec systems that act on short regions of homology. Mol Gen Genet 1984; 195:374–377
    [Google Scholar]
  24. Calos MP, Miller JH. Transposable elements. Cell 1980; 20:579–595
    [Google Scholar]
  25. Cohen SN, Shapiro JA. Transposable genetic elements. Sci Am 1980; 242:236–45
    [Google Scholar]
  26. Iordänescu S. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids. J Bacteriol 1975; 124:597–601
    [Google Scholar]
  27. Iordänescu S. Relationships between cotransducible plasmids in Staphylococcus aureus. J Bacteriol 1977; 129:71–75
    [Google Scholar]
  28. Novick RP, Iordänescu S, Surdeanu M, Edelman I. Transduction-related cointegrate formation between staphylococcal plasmids: a new type of site-specific recombination. Plasmid 1981; 6:159–172
    [Google Scholar]
  29. Anderson ES. Recombination between unrelated bacterial plasmids. Annal Microbiol (Inst Past 1974; 125A:251–259
    [Google Scholar]
  30. El Sohl N, Allignet J, Bismuth R, Buret B, Fouace J-M. Conjugative transfer of staphylococcal antibiotic resistance markers in the absence of detectable plasmid DNA. Antimicrob Agents Chemother 1986; 30:161–169
    [Google Scholar]
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