1887

Abstract

. Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT) O157. However, many organisms other than VT O157, especially other serogroups of and spp., may not ferment sorbitol, and thus may be confused initially with VT O157. Rhamnose is not fermented by VT O157, but is by most sorbitol non-fermenting of other serogroups. Cefixime is a cephalosporin antibiotic that is more active against spp. than against . Inclusion of rhamnose and cefixime in sorbitol-MacConkey agar improves its selectivity for the isolation of VT O157.

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1991-08-01
2022-05-29
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References

  1. Karmali MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989; 2:15–38
    [Google Scholar]
  2. Riley LW, Remis RS, Helgerson SD. et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. TV. Engl J Med 1983; 308:681–685
    [Google Scholar]
  3. Pai CH, Gordon R, Sims HV, Bryan LE. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Ann Intern Med 1984; 101:738–742
    [Google Scholar]
  4. Smith HR, Rowe B, Gross RJ, Fry NK, Scotland SM. Haemorrhagic colitis and verocytotoxin-producing Escherichia coli in England and Wales. Lancet 1987; 1:1062–1065
    [Google Scholar]
  5. Chapman PA, Wright DJ, Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect 1989; 102:439–445
    [Google Scholar]
  6. Karmali MA, Steele BT, Pétrie M, Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cyto-toxin and cytotoxin-producing Escherichia coli in stools. Lancet 1983; 1:619–620
    [Google Scholar]
  7. Karmali MA, Petric M, Lim C, Fleming PC, Arbus GS, Lior H. The association between idiopathic hemolyticuremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis 1985; 151:775–782
    [Google Scholar]
  8. Scotland SM, Rowe B, Smith HR, Willshaw GA, Gross RJ. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic-uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25:237–243
    [Google Scholar]
  9. Spika JS, Parsons JE, Nordenberg D, Wells JG, Gunn RA, Blake PA. Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O157:H7 in a day care center. J Pediatr 1986; 109:287–291
    [Google Scholar]
  10. Martin ML, Shipman LD, Wells JG. et al. Isolation of Escherichia coli Ol 57: H7 from dairy cattle associated with two cases of haemolytic-uraemic syndrome. Lancet 1986; 2:1043
    [Google Scholar]
  11. Chapman PA, Jewes L, Siddons CA, Norman P, George SL. Verotoxin-producing Escherichia coli infections in Sheffield during 1989. PHLS Microbiol Digest 1990; 7:163–166
    [Google Scholar]
  12. Ratnam S, March SB, Sprague WD, Severs D, Sullivan RM. Are humans a source of Escherichia coli O157:H7, the agent of hemorrhagic colitis?. N Engl J Med 1986; 315:1612–1613
    [Google Scholar]
  13. Carter AO, Borczyk AA, Carlson JA. et al. A severe outbreak of Escherichia coli O157: H7 associated hemorrhagic colitis in a nursing home. N Engl J Med 1987; 317:1496–1500
    [Google Scholar]
  14. March SB, Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol 1986; 23:869–872
    [Google Scholar]
  15. Wise R, Andrews JM, Ashby JP, Thornber D. In vitro activity of Bay N 3522, a new cephalosporin, compared with activities of other agents. Antimicrob Agents Chemother 1990; 34:813–818
    [Google Scholar]
  16. Chapman PA. Evaluation of a commercial latex slide test for identifying Escherichia coli O157. J Clin Pathol 1989; 42:1109–1110
    [Google Scholar]
  17. Report Third Annual Report (1988–1989) of the British Paediatric Surveillance Unit; 1989
  18. Wells JG, Davis BR, Wachsmuth IK. et al. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol 1983; 18:512–520
    [Google Scholar]
  19. Kleanthous H, Fry NK, Smith HR, Gross RJ, Rowe B. The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains of Escherichia coli O157. Epidemiol Infect 1988; 101:327–335
    [Google Scholar]
  20. Walker CW, Upson R, Warren RE. Haemorrhagic colitis: detection of verotoxin producing Escherichia coli O157 in a clinical microbiology laboratory. J Clin Pathol 1988; 41:80–84
    [Google Scholar]
  21. Lior H, Borczyk AA. False positive identifications of Escherichia COÜ0157. Lancet 1987; 1:333
    [Google Scholar]
  22. Borczyk AA, Lior H, Ciebin B. False positive identifications of Escherichia coli O157 in foods. Int J Food Microbiol 1987; 4:347–349
    [Google Scholar]
  23. Willshaw GA, Smith HR, Scotland SM, Rowe B. Cloning of genes determining the production of vero cytotoxin by Escherichia coli. J Gen Microbiol 1985; 131:3047–3053
    [Google Scholar]
  24. Calderwood SB, Auclair F, Donohue-Rolfe A, Keusch GT, Mekalanos J J. Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc Natl Acad Sci USA 1987; 84:4364–4368
    [Google Scholar]
  25. Jackson MP, Neill RJ, O’Brien AD, Homes RK, Newland JW. Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933. FEMS Microbiol Lett 1987; 44:109–114
    [Google Scholar]
  26. Strockbine NA, Jackson MP, Sung LM, Holmes RK, O’Brien AD. Cloning and sequencing of the genes for Shiga toxin from Shigella dysenteriae type 1. J Bacteriol 1988; 170:1116–1122
    [Google Scholar]
  27. Willshaw GA, Smith HR, Scotland SM, Field AM, Rowe B. Heterogeneity of Escherichia coli phages encoding vero cytotoxins: comparison of cloned sequences determing VT1 and VT2 and development of specific gene probes. J Gen Microbiol 1987; 133:1309–1317
    [Google Scholar]
  28. Karch H, Meyer T. Evaluation of oligonucleotide probes for identification of Shiga-like-toxin-producing Escherichia coli. J Clin Microbiol 1989; 27:1180–1186
    [Google Scholar]
  29. Chart H, Scotland SM, Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol 1989; 27:285–290
    [Google Scholar]
  30. Chart H, Scotland SM, Smith HR, Rowe B. Antibodies to Escherichia coli O157 in patients with haemorrhagic colitis and haemolytic-uraemic syndrome. J Clin Pathol 1989; 42:973–976
    [Google Scholar]
  31. Ahmed R, Bopp C, Borczyk A, Kasatiya S. Phage-typing scheme for Escherichia coli O157:H7. J Infect Dis 1987; 155:806–809
    [Google Scholar]
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