. Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT) O157. However, many organisms other than VT O157, especially other serogroups of and spp., may not ferment sorbitol, and thus may be confused initially with VT O157. Rhamnose is not fermented by VT O157, but is by most sorbitol non-fermenting of other serogroups. Cefixime is a cephalosporin antibiotic that is more active against spp. than against . Inclusion of rhamnose and cefixime in sorbitol-MacConkey agar improves its selectivity for the isolation of VT O157.


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