1887

Abstract

. The diagnosis of bacterial vaginosis (BV) by direct gram-stained smear of vaginal discharge was evaluated in 90 consecutive patients. Vaginal secretions from 20 healthy female volunteers were also examined. All specimens were examined by culture for growth of and spp. Only 35 (38.9%) of the patients with vaginal discharge satisfied the clinical diagnosis of BV. The vaginal gram-stained smear from all these 35 patients contained typical gardnerella morphotypes characteristic of BV and was isolated from all of them as well as from a further 10 patients who did not satisfy the criteria for diagnosis of BV. The semi-quantitative assessment of gardnerella morphotypes was found to correlate positively with the semi-quantitative assessment of growth by culture (p < 0.0001). There was a strong inverse relationship between the quantity of lactobacillus and gardnerella morphotypes. Direct gram-staining of smears should facilitate the diagnosis of BV for clinicians with minimum need for confirmation by culture, particularly in a third world setting with few or no laboratory facilities.

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1991-08-01
2024-12-05
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References

  1. Gardner HL, Dukes CD. Hemophilus vaginalis vaginitis. Am J Obstet Gynecol 1955; 69:962–976
    [Google Scholar]
  2. Abudu OO, Odugbemi TO, Agboola A. Corynebacterium vaginale and vaginitis. W Afr Med J 1982; 1:19–20
    [Google Scholar]
  3. Spiegel CA, Amsel R, Eschenbach D, Schoenknecht F, Holmes KK. Anaerobic bacteria in nonspecific vaginitis. N Engl J Med 1980; 303:601–607
    [Google Scholar]
  4. Phillips I, Taylor E. Anaerobic curved rods in vaginitis. Lancet 1982; 1:221
    [Google Scholar]
  5. Rotimi VO, Kayode Y, Oji CO, Dosunmu-Ogunbi O. The bacterial flora of non-specific vaginitis. Afr J Sex Trans Dis 1984; 2:34–37
    [Google Scholar]
  6. Rotimi VO, Oji GO. Gardnerella vaginalis in non-specific vaginitis: its occurrence and antimicrobial susceptibility. Afr J Clin Microbiol 1984; 1:47–54
    [Google Scholar]
  7. Spiegel CA, Eschenbach DA, Amsel R, Holmes KK. Curved anaerobic bacteria in bacterial (nonspecific) vaginosis and their response to antimicrobial therapy. J Infect Dis 1983; 148:817–822
    [Google Scholar]
  8. Spiegel CA, Amsel R, Holmes KK. Diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid. J Clin Microbiol 1983; 18:170–177
    [Google Scholar]
  9. Chen KCS, Amsel R, Eschenbach DA, Holmes KK. Biochemical diagnosis of vaginitis: determination of diamines in vaginal fluid. J Infect Dis 1982; 145:337–345
    [Google Scholar]
  10. Bump RC, Buesching WJ. Bacterial vaginosis in virginal and sexually active adolescent females: evidence against exclusive sexual transmission. Am J Obstet Gynecol 1988; 158:935–939
    [Google Scholar]
  11. Dunkelberg WE. Diagnosis of Hemophilus vaginalis vaginitis by gram-stained smears. Am J Obstet Gynecol 1965; 91:998–1000
    [Google Scholar]
  12. Balsdon MJ, Taylor GE, Pead L, Maskell R. Corynebacterium vaginale vaginitis: a controlled trial of treatment. Lancet 1980; 1:501–504
    [Google Scholar]
  13. Pheifer TA, Forsyth PS, Durfee MA, Pollock HM, Holmes KK. Nonspecific vaginitis: role of Haemophilus vaginalis and treatment with metronidazole. N Engl J Med 1978; 298:1429–1434
    [Google Scholar]
  14. Oji GO, Rotimi VO. Gardnerella vaginalis in non-specific vaginitis: isolation and identification. Afr J Clin Microbiol 1984; 1:39–46
    [Google Scholar]
  15. Gillies RR, Dodds TC. Bacteriology illustrated. Edinburgh: Churchill Livingstone; 1976
    [Google Scholar]
  16. Eschenbach DA, Hilliers S, Critchlow C, Stevens C, DeRonen T, Holmes KK. Diagnosis and clinical manifestations of bacterial vaginosis. Am J Obstet Gynecol 1988; 158:819–828
    [Google Scholar]
  17. Lennette EH, Balows A, Hausier WJ, Shadomy H J. eds Manual of clinical microbiology, 4th. Washington, DC: American Society for Microbiology; 1985
    [Google Scholar]
  18. Totten PA, Amsel RA, Hale J, Piot P, Holmes KK. Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis. J Clin Microbiol 1982; 15:141–147
    [Google Scholar]
  19. Popp W. The diagnosis and treatment of mixed anaerobic vaginal discharges. Geburtshilfe Frauenheilkd 1977; 37:432–437
    [Google Scholar]
  20. Thomason JL, Schreckenberger PC, Spellacy WN, Ruff LJ, LeBeau LJ. Clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods. J Infect Dis 1984; 149:801–809
    [Google Scholar]
  21. Amsel R, Totten PA, Spiegel CA, Chen KCS, Eschenbach D, Holmes KK. Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med 1983; 74:14–22
    [Google Scholar]
  22. Spiegel CA, Amsel R, Eschenbach D, Schoenknecht F, Holmes KK. Anaerobic bacteria in nonspecific vaginitis. N Engl J Med 1980; 303:601–607
    [Google Scholar]
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