1887

Abstract

Summary

Growth of and was monitored during early stages of single (mono-) and mixed intra-abdominal infection in a rat fibrin clot model. When and were together involved in the infection, numbers increased about 6h after an initial decline. This increase was not found with mono-infections. The numbers of increased rapidly in both mono- and mixed infections and stayed high for several days, but only mixed infection resulted in abscesses that persisted for more than 7 days. Macrophages, the main component of the peritoneal cellular defence mechanism, were outnumbered by polymorphonuclear leucocytes during the first 6 h of infection. Further characterisation of the macrophage population by means of monoclonal antibodies showed a shift from resident to exudate macrophages as the result of influx of the latter.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-35-1-18
1991-07-01
2023-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/35/1/medmicro-35-1-18.html?itemId=/content/journal/jmm/10.1099/00222615-35-1-18&mimeType=html&fmt=ahah

References

  1. Swenson RM, Lorber B, Michaelson TC, Spaulding EH. The bacteriology of intra-abdominal infections. Arch Surg 1974; 109:398–399
    [Google Scholar]
  2. Swenson RM, Lorber B. Clindamycin and carbenicillin in treatment of patients with intra-abdominal and female genital tract infections. J Infect Dis 1977; 135:S40–S45
    [Google Scholar]
  3. Onderdonk AB, Bartlett JG, Louie T, Sullivan-Seigler N, Gorbach SL. Microbial synergy in experimental intraabdominal abscess. Infect Immun 1976; 13:22–26
    [Google Scholar]
  4. Rotstein OD, Pruett TL, Simmons RL. Lethal microbial synergism in intra-abdominal infections. Arch Surg 1985; 120:146–151
    [Google Scholar]
  5. Verweij-Van Vught AMJJ, Namavar F, Sparrius M, Vel WAC, MacLaren DM. Pathogenic synergy between Escherichia coli and Bacteroides fragilis: studies in an experimental mouse model. J Med Microbiol 1985; 19:325–331
    [Google Scholar]
  6. Rotstein OD, Kao J. The spectrum of Escherichia coli-Bacteroides fragilis pathogenic synergy in an intraabdominal infection model. Can J Microbiol 1988; 34:352–357
    [Google Scholar]
  7. Rotstein OD, Kao J, Houston K. Reciprocal synergy between Escherichia coli and Bacteroides fragilis in an intraabdominal infection model. J Med Microbiol 1989; 29:269–276
    [Google Scholar]
  8. Dunn DL, Barke RA, Ewald DC, Simmons RL. Macrophages and translymphatic absorption represent the first line of host defence of the peritoneal cavity. Arch Surg 1987; 122:105–110
    [Google Scholar]
  9. Ahrenholz DH, Simmons RL. Fibrin in peritonitis. I. Beneficial and adverse effects of fibrin in experimental E. coli peritonitis. Surgery 1980; 88:41–47
    [Google Scholar]
  10. Vel WAC, Namavar F, Verweij-Van Vught AMJJ, Pubben ANB, MacLaren DM. Interactions between polymorphonuclear leucocytes, Bacteroides sp., and Escherichia coli: their role in the pathogenesis of mixed infection. J Clin Pathol 1986; 39:376–382
    [Google Scholar]
  11. Verweij-Van Vught AMJJ, Namavar F, Vel WAC, Sparrius M, MacLaren DM. Pathogenic synergy between Escherichia coli and Bacteroides fragilis or B. vulgatus in experimental infections: a non-specific phenomenon. J Med Microbiol 1986; 21:43–47
    [Google Scholar]
  12. Namavar F, Verweij-Van Vught AMJJ, Bal M, MacLaren DM. Effect of Bacteroides fragilis cellular components on chemotactic activity of polymorphonuclear leukocytes towards Escherichia coli. J Med Microbiol 1987; 24:119–124
    [Google Scholar]
  13. Namavar F, Verweij AMJJ, Bal M, van Steenbergen TJM, de Graaff J, MacLaren DM. Effect of anaerobic bacteria on killing of Proteus mirabilis by human polymorphonuclear leukocytes. Infect Immun 1983; 40:930–935
    [Google Scholar]
  14. Dunn DL, Rotstein OD, Simmons RL. Fibrin in peritonitis. IV. Synergistic intraperitoneal infection caused by Escherichia coli and Bacteroides fragilis within fibrin clots. Arch Surg 1984; 119:139–144
    [Google Scholar]
  15. Dijkstra CD, Döpp EA, Joling P, Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED 3. Immunology 1985; 54:589–599
    [Google Scholar]
  16. Beelen RHJ, Eestermans IL, Döpp EA, Dijkstra CD. Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: expression of recognized antigens in different stages of differentiation. Transplant Proc 1987; 19:3166–3170
    [Google Scholar]
  17. Bos HJ, Meyer F, de Veld JC, Beelen RHJ. Peritoneal dialysis fluid induces change of mononuclear phagocyte proportions. Kidney Int 1989; 36:20–26
    [Google Scholar]
  18. MacLaren DM, Namavar F, Verweij-Van Vught AMJJ, Vel WAC, Kaan JA. Pathogenic synergy: mixed intraabdominal infections. Antonie Van Leeuwenhoek 1984; 50:775–787
    [Google Scholar]
  19. Rotstein OD, Kao J. Prevention of intra-abdominal abscesses by fibrinolysis using recombinant tissue plasminogen activator. J Infect Dis 1988; 158:766–772
    [Google Scholar]
  20. McRitchie DI, Cummings D, Rotstein OD. Delayed administration of tissue plasminogen activator reduces intraabdominal abscess formation. Arch Surg 1989; 124:1406–1410
    [Google Scholar]
  21. Miles AA, Miles EM, Burke J. The value and duration of defence reactions of the skin to the primary lodgement of bacteria. Br J Exp Pathol 1957; 38:79–96
    [Google Scholar]
  22. Shapiro M, Sacks T. A decisive period in cefoxitin prophylaxis of experimental synergistic wound infection produced by Bacteroides fragilis and Escherichia coli. Isr J Med Sci 1982; 18:863–865
    [Google Scholar]
  23. Onderdonk AB, Cisneros RL, Crabb JH, Finberg RW, Kasper DL. Intraperitoneal host cellular responses and in vivo killing of Bacteroides fragilis in a bacterial containment chamber. Infect Immun 1989; 57:3030–3037
    [Google Scholar]
  24. Dunn DL, Barke RA, Knight NB, Humphrey EW, Simmons RL. Role of resident macrophages, peripheral neutrophils, and translymphatic absorption in bacterial clearance from the peritoneal cavity. Infect Immun 1985; 49:257–264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-35-1-18
Loading
/content/journal/jmm/10.1099/00222615-35-1-18
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error