1887

Abstract

Summary

The kinetics of adherence of single isolates of and to catheters made of polyvinyl chloride (PVC), Teflon, siliconised latex, polyurethane and Vialon was evaluated by a radiometric assay. Radiolabelled bacteria (10 cfu/ml) were incubated in vials containing 1-cm lengths of catheter for up to 3 days. The peak of maximal adherence to each biomaterial was reached after 24 h for and after 72 h for the other strains. Bacterial adherence to PVC and siliconised latex was significantly higher (2–6 times; p≪0.05) than to the other biomaterials for all the strains. The lowest values of adherence were observed with polyurethane and Vialon for the staphylococci but with Teflon for and . Bacterial viability and growth was evaluated in eluates obtained from incubation of segments of each catheter in buffer for 24 h. None of the eluates affected the viability of the staphylococci. However, all of them, significantly increased the growth of and with the exception of the eluate from siliconised latex, in which the inoculum count was reduced to an undetectable level for . We conclude that bacterial adherence to catheters may depend in part on the nature of the biomaterial and that certain substances eluted from the catheters may affect the viability and growth of different micro-organisms.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-34-6-349
1991-06-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/34/6/medmicro-34-6-349.html?itemId=/content/journal/jmm/10.1099/00222615-34-6-349&mimeType=html&fmt=ahah

References

  1. Cleri D. J., Corrado M. L., Seligman S. J. Quantitative culture of intravenous catheters and other intravascular inserts. J Infect Dis 1980; 141:781–787
    [Google Scholar]
  2. Maki D. G., Goldman D. A., Rhame F. S. Infection control in intravenous therapy. Ann Intern Med 1973; 79:867–887
    [Google Scholar]
  3. Maki D. G. Nosocomial bacteriemia: an epidemiologic over view. Am J Med 1981; 70:719–732
    [Google Scholar]
  4. Peters G., Locci R., Pulverer G. Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. J Infect Dis 1982; 146:479–482
    [Google Scholar]
  5. Welch G. W., McKeel D. W., Silverstein P., Walker H. L. The role of catheter composition in the development of thrombophlebitis. Surg Gyn Obstet 1974; 138:421–424
    [Google Scholar]
  6. Pascual A., Fleer A., Westerdaal N. A. C., Verhoef J. Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro. Eur J Clin Microbiol 1986; 5:518–522
    [Google Scholar]
  7. Franson T. R., Sheth N. K., Menon L., Sohnle P. G. Persistent in vitro survival of coagulase-negative staphylococci adherent to intravascular catheters in the absence of conventional nutrients. J Clin Microbiol 1986; 24:559–561
    [Google Scholar]
  8. Verhoef J., Peterson P. K., Quie P. G. Kinetics of staphylococci opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using (3H)-thymidine labelled bacteria. J Immunol Methods 1977; 14:303–310
    [Google Scholar]
  9. Ashkenazi S., Mirelman D. Adherence of bacteria to pediatric intravenous catheters and needles and its relation to phlebitis in animals. Pediatr Res 1984; 18:1361–1366
    [Google Scholar]
  10. Wallenstein S., Zucker C. L., Fleiss J. L. Some statistical methods used in circulation research. Circ Res 1980; 47:1–9
    [Google Scholar]
  11. Sheth N. K., Rose H. D., Franson T. R., Buckmire F. L. A., Sohnle P. G. in vitro quantitative adherence of bacteria to intravascular catheters. J Surg Res 1983; 34:213–218
    [Google Scholar]
  12. Hogt A. H., Dankert J., Hulstaert C. E., Feijen J. Cell surface characteristics of coagulase-negative staphylococci and their adherence to fluorinated poly (ethylenepropylene). Infect Immun 1986; 51:294–301
    [Google Scholar]
  13. Kristinsson K. G. Adherence of staphylococci to intravascular catheters. J Med Microbiol 1989; 28:249–257
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-34-6-349
Loading
/content/journal/jmm/10.1099/00222615-34-6-349
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error