Conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. Whole-cell protein electrophoresis was then performed with extracts of each strain. Allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. Organisms identified conventionally as and formed homogeneous groups by protein electrophoresis. There was evidence for heterogeneity amongst strains identified as (two groups, including and . However, aberrant strains were allocated to the groups, leaving a homogeneous group, and if were re-instated as a species, the remaining strains could be divided into two groups. Of the anaerobic gram-positive cocci in the National Collection of Type Cultures deposited by Hare, Group IV is , Group IX is and Groups I, III and VIII appear to be related to the butyrate-producing species and , but are strongly saccharolytic.


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