1887

Abstract

Summary

A collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (GPAC) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. The methods used were: gas-liquid chromatography (GLC) for the detection of volatile fatty acids (VFAs); determination of the pre-formed enzyme profile with a commercially available kit, ATB 32A; microscopic appearance; colonial morphology; and antibiotic sensitivity tests. Strains were placed in one of five VFA groups according to their GLC profile; 96% of strains were further assigned to 12 groups by their enzyme profile. There was >99% agreement between the two methods.Of 111 clinical strains in the VFA-negative group, 110 gave one of three distinct enzyme profiles corresponding to and . The assignment of strains to groups based on their microscopic appearance and colonial morphology agreed well with groupings according to enzyme profile. Identification of butyrate-producing GPAC was unsatisfactory because it relied heavily on the enzyme profile; testing for indole production was of limited discriminative value. Most strains of and were very similar in enzyme profile, microscopic appearance and colonial morphology, but a sub-group of could be distinguished. A further indole-positive group corresponding to Hare group III was also noted. Strains of and were very similar, but easily distinguished from other butyrateproducing GPAC. However, 45% of the butyrate-producing cocci could not be assigned to recognised species; most of these were assigned to one of two new groups, the ADH group and the bGAL group, by their enzyme profile, microscopic appearance and smell. Four strains that produced a terminal VFA peak of valeric acid formed a new group designated ‘ivoricus’. Reliable features for the identification of were GLC (all GPAC that produced caproic acid were identified as ), enzyme profile and sensitivity to SPS. Two clinical strains that produced caproic acid were identified as Hare group VIII; they were distinguished from by their enzyme profile and colonial morphology.A phenotypic classification based on GLC and enzyme profile is presented, with a method for the identification of most strains of GPAC within 48 h of primary isolation.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-34-5-295
1991-05-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/34/5/medmicro-34-5-295.html?itemId=/content/journal/jmm/10.1099/00222615-34-5-295&mimeType=html&fmt=ahah

References

  1. Watt B., Jack E. P. What are anaerobic cocci?. J Med Microbiol 1977; 10:461–468
    [Google Scholar]
  2. Brookl Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals. J Clin Microbiol 1988; 26:1181–1188
    [Google Scholar]
  3. Gorbach S. L. Anaerobic cocci. In Mandell G. L., Douglas R. G., Bennett J. E. (eds) Principles and practice of infectious diseases 2nd edn New York, John Wiley: 19851373–1375
    [Google Scholar]
  4. Wren M. W. D., Baldwin A. W. F., Eldon C. P., Sanderson P. J. The anaerobic culture of clinical specimens: a 14-month study. J Med Microbiol 1977; 10:49–61
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Moore W. E. C. Anaerobe laboratory manual. 4th edn Blacksburg, Virginia Polytechnic Institute and State Univerity 1977
    [Google Scholar]
  6. Sutter V. L., Citron D. M., Edelstein M. A. C., Finegold S. M. Wadsworth anaerobic bacteriology manual. v4th edn Los Angeles, Star Publishing Co 1985
    [Google Scholar]
  7. Parker M. T. The anaerobic gram-positive cocci. In Wilson G. (eds) Topley and Wilson’s principles of bacteriology, virology and immunity 7th edn vol 2 London: Edward Arnold; 1983238–239
    [Google Scholar]
  8. Holdeman Moore L. V., Johnson J. L., Moore W. E. C. Genus Peptococcus and Genus Pep tost reptococcus. In Sneath P. H. A. (ed) Bergey’s Manual of systematic bacteriology, vol 2 Baltimore: Williams and Wilkins; 19861082–1092
    [Google Scholar]
  9. Ezaki T., Yamamoto N., Ninomiya K., Suzuki S., Yabuuchi E. Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. Int J Syst Bact 1983; 33:683–698
    [Google Scholar]
  10. Huss V. A. R., Festl H., Schleifer K. H. Nucleic acid hybridization studies and deoxyribonucleic acid base compositions of anaerobic, gram-positive cocci. Int J Syst Bact 1984; 34:95–101
    [Google Scholar]
  11. Ezaki T., Yabuuchi E. Oligopeptidase activity of gram-positive anaerobic cocci used for rapid identification. J Gen Appl Microbiol 1985; 31:255–265
    [Google Scholar]
  12. Murdoch D. A., Mitchelmore I. J., Tabaqchali S. Identification of gram-positive anaerobic cocci by use of systems for detecting pre-formed enzymes. J Med Microbiol 1988; 25:289–293
    [Google Scholar]
  13. Murdoch D. A., Mitchelmore I. J., Nash R. A., Hardie J. M. Tabaqch ali S. Pre-formed enzyme profiles of reference strains of gram-positive anaerobic cocci. J Med Microbiol 1988; 27:65–70
    [Google Scholar]
  14. Wren M. W. D., Eldon C. P., Dalin G. H. Novobiocin and the differentiation of peptococci and peptostreptococci. J Clin Pathol 1977; 30:620–622
    [Google Scholar]
  15. Cato E. P., Johnson J. L., Hash D. E., Holdeman L. V. Synonomy of Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957 with Peptostreptococcus micros (Prevot 1933) Smith 1957 and electrophoretic differentiation of Peptostreptococcus micros from Peptococcus magnus (Prevot 1933) Holdeman and Moore 1972. Inst J Syst Bact 1983; 33:207–210
    [Google Scholar]
  16. Harpold D. J., Wasilauskus B. L. Rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography. J Clin Microbiol 1987; 25:996–1001
    [Google Scholar]
  17. Holdeman L. V., Moore W. E. C. New genus, Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human feces. Int J Syst Bact 1974; 24:260–277
    [Google Scholar]
  18. Wiggins R. J., Wilks M., Tabaqchali S. Analysis by gas liquid chromatography of production of volatile fatty acids by anaerobic bacteria grown on solid medium. J Clin Pathol 1985; 38:933–936
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-34-5-295
Loading
/content/journal/jmm/10.1099/00222615-34-5-295
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error