Human polymorphonuclear leucocytes (PMNL) underwent several changes in response to challenge with , namely (1) an increase in oxygen uptake, (2) changes in membrane electrical properties, and (3) increased transport of chloride ions (C1) across the membrane. Mean oxygen consumption and C1 uptake by PMNL were stimulated by both pilate (P) and non-pilate (P) gonococci, although the levels were much reduced in the presence of P organisms. P gonococci also initiated low levels of polarisation or depolarisation in contrast with P cells, which caused hyperpolarisation followed by depolarisation in the PMNL. Most of the strains showed these patterns. High performance liquid chromatography of extracts of unstimulated PMNL and of PMNL challenged with gonococci confirmed production of hypochlorous acid (HOC1) in the leucocyte. Furthermore, addition of radiolabelled C1 to the PMNLs showed that some of the C1 taken up by the cells in response to gonococcal challenge was incorporated into the HOC1, suggesting a direct relationship between stimulation of C1 uptake and production of active chlorine compounds in the leucocyte.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error