1887

Abstract

Summary

A collection of 223 strains of non-typable was assembled. Most strains were isolated from hospital in-patients in North East Scotland between 1984 and 1989. These isolates were examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole cell polypeptides. Variability was assessed in terms of apparent molecular weight differences between the protein profiles. Isolates were grouped on the basis of Dice coefficients of similarity and assigned to clones. Of the 223 strains, 147 were unique; the remaining strains were assigned to multi-member clones of which 13 clones had two members, one clone had three members, three clones had four members, three clones had five members, two clones had six members and one clone had eight members. The longest time interval between isolation of clonally related strains virtually equalled the limits of the study. Isolates from a childrens hospital were significantly less diverse than those from patients in adult wards.

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1991-05-01
2024-12-14
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References

  1. Selander R. K., Musser J. M., Caugant D. A., Gilmour M. N., Whittam T. S. Population genetics of pathogenic bacteria. Microb Pathog 1987; 3:1–7
    [Google Scholar]
  2. Eisenstein B. A. New molecular techniques for microbial epidemiology and the diagnosis of infectious disease. J Infect Dis 1990; 161:595–602
    [Google Scholar]
  3. Hartl D. L., Dykhuizen D. E. The population genetics of Esche richia coli. Annu Rev Genet 1984; 18:31–68
    [Google Scholar]
  4. Dyas A., George R. H. Ten years’ experience of Haemophilus influenzae infection at Birmingham Children’s Hospital. J Infect 1986; 13:179–185
    [Google Scholar]
  5. Turk D. C. The pathogenicity of Haemophilus influenzae. J Med Microbiol 1984; 18:1–16
    [Google Scholar]
  6. Murphy T. F., Apicella M. A. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev Infect Dis 1987; 9:1–15
    [Google Scholar]
  7. Musser J. M., Granoff D. M., Pattison P. E., Selander R. K. A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci USA 1985; 82:5078–5082
    [Google Scholar]
  8. Musser J. M., Kroll J. S., Granoff D. M. Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis 1990; 12:75–111
    [Google Scholar]
  9. Paterson A. J., MacSween K. F., Pennington T. H. Haemophilus influenzae subtyping by SDSPAGE of whole-cell polypeptides. Epidemiol Infect 1987; 99:179–189
    [Google Scholar]
  10. Porras O., Caugant D. A., Gray B., Lagergard T., Levin B. R., Svanborg-Eden C. Difference in structure between type b and nontypable Haemophilus influenzae populations. Infect Immun 1986; 53:79–89
    [Google Scholar]
  11. Musser J. M., Barenkamp S. J., Granoff D. M., Selander R. K. Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun 1986; 52:183–191
    [Google Scholar]
  12. Pennington T. H., Freebaim E. M. Subtyping of Haemophilus influenzae type b strains from Europe and North America by SDS-PAGE of whole-cell polypeptides:the geographical distribution of subtypes. Epidemiol Infect 1989; 102:11–19
    [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  14. Murphy T. F., Dudas K. C., Mylotte J. M., Apicella M. A. A subtyping system for nontypable Haemophilus influenzae based on outer-membrane proteins. J Infect Dis 1983; 147:838–846
    [Google Scholar]
  15. Dice L. R. Measures of the amount of ecologic association between species. Ecology 1945; 26:297–302
    [Google Scholar]
  16. Murphy T. F., Bartos L. C. Purification and analysis with monoclonal antibodies of P2, the major outer membrane protein of nontypable Haemophilus influenzae. Infect Immun 1988; 56:1084–1089
    [Google Scholar]
  17. Fisher R. A., Corbet A. S., Williams C. B. The relation between the number of species and the number of individuals in a random sample of an animal population. J Animal Ecology 1943; 12:42–58
    [Google Scholar]
  18. Turk D. C. Haemophilus influenzae. Public Health Laboratory Service Monograph Series No. 17 London, HMSO: 1982; 156
    [Google Scholar]
  19. Spinola S. M., Peacock J., Denny F. W., Smith D. L., Cannon J. G. Epidemiology of colonization by nontypable Haemophilus influenzae in children: a longitudinal study. J Infect Dis 1986; 154:100–109
    [Google Scholar]
  20. Loeb M. R., Smith D. H. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infect Immun 1980; 30:709–717
    [Google Scholar]
  21. Porras O., Caugant D. A., Lagergard T., Svanborg-Eden C. Application of multilocus enzyme gel electrophoresis to Haemophilus influenzae. Infect Immun 1986; 53:71–78
    [Google Scholar]
  22. Weinberg G. A., Ghafoor A., Ishaq Z. Clonal analysis of Haemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections. J Infect Dis 1989; 160:634–643
    [Google Scholar]
  23. Selander R. K., Caugant D. A., Whittam T. S. Genetic structure and variation in natural populations of Escherichia coli. In Neidhart F. C. (ed) Escherichia coli and Salmonella typhimu-rium: cellular and molecular biology Washington DC: American Society for Microbiology; 19871625–1668
    [Google Scholar]
  24. Caugant D. A., Bovre K. Gaustad P et al., Multilocus genotypes determined by enzyme electrophoresis of Neisseria meningitidis isolated from patients with systemic disease and from healthy carriers. J Gen Microbiol 1986; 132:641–652
    [Google Scholar]
  25. Kimura M. The neutral theory of molecular evolution. Cam bridge Cambridge University Press; 1983
    [Google Scholar]
  26. Leigh-Brown A. J., Langley C. H. Reevaluation of level of genic heterozygosity in natural population of Drosophila melano-gaster by two-dimensional electrophoresis. Proc Natl Acad Sci USA 1979; 76:2381–2384
    [Google Scholar]
  27. Neel J. V. Average locus differences in mutability related to protein ‘class’. A hypothesis. Proc Natl Acad Sci USA 1990; 87:2062–2066
    [Google Scholar]
  28. Stuy J. H. Transfer of genetic information within a colony of Haemophilus influenzae. J Bacterial 1985; 162:1–4
    [Google Scholar]
  29. Rowji P., Gromkova R., Koomhof H. Genetic transformation in encapsulated clinical isolates of Haemophilus influenzae type b. J Gen Microbiol 1989; 135:2775–2782
    [Google Scholar]
  30. Musser J. M., Kroll J. S., Moxon E. R., Selander R. K. Clonal population structure of encapsulated Haemophilus influenzae. Infect Immun 1988; 56:1837–1845
    [Google Scholar]
  31. Zei G., Matessi R. G., Siri E., Moroni A, Cavalli-Sforza L. Surnames in Sardinia I. Fit of frequency distributions for neutral alleles and genetic population structure. Ann Human Genet 1983; 47:329–352
    [Google Scholar]
  32. Karlin S., McGregor J. The number of mutant forms maintained in a population. In Le Cam L. M., Neyman J. (eds) Proc Fifth Berkeley Symp Math Stat Prob Berkeley: University of California Press; 1967; 4415–438
    [Google Scholar]
  33. Zei G., Piazza A., Moroni A, Cavalli-Sforza L. L. Surnames in Sardinia III. The spatial distribution of surnames for testing neutrality of genes. Ann Hum Genet 1986; 50:169–180
    [Google Scholar]
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