The association of haemagglutination and adhesion with lipopolysaccharide of serotype 1 Free

Abstract

Summary

In this study the ability of strains of serotype 1 to agglutinate mammalian erythrocytes is attributed to the polysaccharide fraction of bacterial-cell lipopolysaccharide (LPS). LPS obtained from a rough, mutant strain of serotype 1 lacking the O-antigen polysaccharide side-chain, did not agglutinate erythrocytes, clearly demonstrating a link between O-antigen polysaccharides and haemagglutinating activity (HA). Strains of serotype 1 adhered well to cultured Henle Intestinal 407 cells, whereas rough strains adhered poorly. Pre-treatment of bacteria with LPS-specific antisera inhibited both HA and binding to cultured human-intestinal cells. The contribution of the polysaccharide side-chain and its associated HA–which appear to facilitate binding to cultured cells–to bacterial attachment to colonocytes and to the pathogenesis of shigellosis needs to be confirmed in animal studies.

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1991-05-01
2024-03-29
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References

  1. Binns M. M. Molecular genetics of virulence in Shigella. Microbiol Sci 1985; 2:275–278
    [Google Scholar]
  2. Lindberg A. A., Haeggman S., Karlsson K., Cam P. D., Trach D. D. The humoral antibody response to Shigella dysenteriae type 1 infection, as determined by ELISA. Bull WHO 1984; 62:597–606
    [Google Scholar]
  3. Levine M. M., Kaper J. B., Black R. E., Clements M. L. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol Rev 1983; 47:510–550
    [Google Scholar]
  4. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 1981; 143:325–345
    [Google Scholar]
  5. Chan R., Acres S. D., Costerton J. W. Morphological examination of cell surface structures of enterotoxigenic strains of Escherichia coli. Can J Microbiol 1984; 30:451–460
    [Google Scholar]
  6. McSweegan E., Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun 1986; 53:141–148
    [Google Scholar]
  7. Izhar M., Nuchamowitz Y., Mirelman D. Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesin. Infect Immun 1982; 35:1110–1118
    [Google Scholar]
  8. Pal T., Hale T. L. Plasmid-associated adherence of Shigella flexneri in a HeLa cell model. Infect Immun 1989; 57:2580–2582
    [Google Scholar]
  9. Qadri F., Haq S., Ciznar I. Hemagglutinating properties of Shigella dysenteriae type 1 and other Shigella species. Infect Immun 1989; 57:2909–2911
    [Google Scholar]
  10. Haider K., Azad A. K., Qadri F., Nahar S., Ciznar I. Role of plasmids in virulence-associated attributes and in O-antigen expression in Shigella dysenteriae type 1 strains. J Med Microbiol 1989; 33:1–9
    [Google Scholar]
  11. Sereny B. Experimental Shigella keratoconjunctivitis. Acta Microbiol Acad Sci Hung 1955; 2:293–296
    [Google Scholar]
  12. Qadri F., Hossain S. A. Ciznar I et al., Congo red binding and salt aggregation as indicators of virulence in Shigella species. J Clin Microbiol 1988; 26:1343–1348
    [Google Scholar]
  13. Goldhar J., Penny R., Golecki J. R., Hoschutzky H., Jann B., Jann K. Nonfimbrial, mannose-resistant adhesins from uropath-ogenic Escherichia coli 083:K1:H4 and 014:K?:H11. Infect Immun 1987; 55:1837–1842
    [Google Scholar]
  14. Salit I. E., Gotschlich E. C. Hemagglutination by purified type 1 Escherichia coli pili. J Exp Med 1977; 146:1169–1181
    [Google Scholar]
  15. Johnston K. H., Gotschlich E. C. Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol 1974; 119:250–257
    [Google Scholar]
  16. Oaks E. V., Hale T. L., Formal S. B. Serum immune response to Shigella protein antigens in Rhesus monkeys and humans infected with Shigella spp. Infect Immun 1986; 53:57–63
    [Google Scholar]
  17. Westphal O., Jann K. Bacterial lipopolysaccharides: Extraction with phenol-water and further applications of the procedure. In Whistler R. L. (ed) Methods in carbohydrate chemistry vol 5 New York: Academic Press; 196583–91
    [Google Scholar]
  18. Moll A., Kusecek B. Pluschke G et al., A reexamination of the Ol lipopolysaccharide antigen group of Escherichia coli. Infect Immun 1986; 53:257–263
    [Google Scholar]
  19. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28:350–356
    [Google Scholar]
  20. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254
    [Google Scholar]
  21. Weissbach A., Hurwitz J. The formation of 2-keto-3-deoxyhep-tonic acid in extracts of Escherichia coli B. J Biol Chem 1959; 234:705–709
    [Google Scholar]
  22. Piotrowicz B. I., Edlin S. E., MaCartney A. C. A sensitive chromogenic limulus amoebocyte lysate micro-assay for detection of endotoxin in human plasma and in water. Zentralbl Bakteriol Mikrobial Hyg 1985a; 260:108–112
    [Google Scholar]
  23. Svennerholm L. Quantative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta 1957; 24:604–611
    [Google Scholar]
  24. Weeke B. Crossed immunoelectrophoresis. In Axelsen N. H., Kroll J., Weeke B. (eds) A manual of quantitative immunoelectrophoresis Oslo: Universitetsoforlaget; 197347–56
    [Google Scholar]
  25. Ouchterlony O Diffusion-in-gel methods for immunological analysis (II). Prog Allergy 1962; VI:30–154
    [Google Scholar]
  26. Mroczenski-Wildey MJ Di, Fabio JL, , Cabello F. C. Invasion and lysis of HeLa cell monolayers by Salmonella typhi: the role of lipopolysaccharide. Microb Pathog 1989; 6:143–152
    [Google Scholar]
  27. Cohen P. S., Rossoll R., Cabelli V. J., Yang S.-L., Laux D. C. Relationship between the mouse colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein. Infect Immun 1983; 40:62–69
    [Google Scholar]
  28. Bradley S. G. Cellular and molecular mechanisms of action of bacterial endotoxins. AnnuRev Microbiol 1979; 33:67–94
    [Google Scholar]
  29. Watanabe H., Nakamura A., Timmis K. N. Small virulence plasmid of Shigella dysenteriae 1 strain W30864 encodes a 41, 000-dalton protein involved in formation of specific lipopolysaccharide side chains of serotype 1 isolates. Infect Immun 1984; 46:55–63
    [Google Scholar]
  30. Lindahl M., Wadstrom T. K99 surface hemagglutinin of enterotoxigenic E. coli recognizes terminal N-acetylgalac-tosamine and sialic acid residues of glycophorin and other complex glycoconjugates. Vet Microbiol 1984; 9:249–257
    [Google Scholar]
  31. Lindahl M., Faris A., Wadstrom T. Colonisation factor antigen on enterotoxigenic Escherichia coli is a sialic-specific lectin. Lancet 1982; 2:280
    [Google Scholar]
  32. Ellen R. P., Fillery E. D., Chan K. H., Grove D. A. Sialidase-enhanced lectin-like mechanism for Actinomyces viscosus and Actinomyces naeslundii hemagglutination. Infect Immun 1980; 27:335–343
    [Google Scholar]
  33. Chitnis D. S., Sharma K. D., Kamat R. S. Role of somatic antigen of Vibrio cholerae in adhesion to intestinal mucosa. J Med Microbiol 1982; 15:53–61
    [Google Scholar]
  34. Cohen P. S., Arruda J. C., Williams T. J., Laux D. C. Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus. Infect Immun 1985; 48:139–145
    [Google Scholar]
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