1887

Abstract

Summary

Pyrolysis mass spectrometry (PMS) was evaluated for the epidemiological typing of coagulase-negative staphylococci (CNS) in situations in which it was necessary to distinguish between repeated isolation of the same strain from a single patient (genuine infection) and coincidental isolation of unrelated strains (contamination). Thirteen CNS isolates were examined, consisting of five pairs, each pair isolated from a single patient, and three unrelated strains. PMS analysis gave results equivalent to a conventional typing system comprising antibiogram, biotype and plasmid profile analysis. Both methods facilitate differentiation between genuine infection with CNS and the isolation of contaminants. The speed, reproducibility, versatility and relatively low cost of PMS suggest that it may be a valuable new technique for the epidemiological typing of CNS in routine clinical settings.

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/content/journal/jmm/10.1099/00222615-34-5-245
1991-05-01
2024-04-16
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References

  1. Gutteridge C. S., Norris J. R. The application of pyrolysis techniques to the identification of micro-organisms. J Appl Bacterial 1979; 47:5–43
    [Google Scholar]
  2. Shute L.A., Berkeley R. C. W., Norris J. R., Gutteridge C. S. Pyrolysis mass-spectrometry in bacterial systems. In Goodfellow M., Minnikin D. E. Chemical methods in bacterial systematics (The Society for Applied Bacteriology Technical Series No. 20) London: Academic Press; 198595–114
    [Google Scholar]
  3. Magee J. T., Hindmarch J. M., Burnett I. A., Pease A. Epidemio logical typing of Streptococcus pyogenes by pyrolysis mass spectrometry. J Med Microbiol 1989; 30:273–278
    [Google Scholar]
  4. Freeman R., Goodfellow M., Gould F. K., Hudson S. J., Lightfoot N. F. Pyrolysis-mass spectrometry (Py-MS) for the rapid epidemiological typing of clinically significant bacterial pathogens. J Med Microbiol 1990; 32:283–286
    [Google Scholar]
  5. Brown D., Blowers R. Disc methods of sensitivity testing and other semiquantitative methods. In Reeves D. S., Phillips I., Williams J. D., Wise R. Laboratory methods in antimicrobial chemotherapy; Edinburgh, Churchill Livingstone: 1978; 30
    [Google Scholar]
  6. Bimboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
    [Google Scholar]
  7. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarosegel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacterial 1976; 127:1529–1537
    [Google Scholar]
  8. Sokal R. R., Michener C. D. A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 1958; 38:1409–1438
    [Google Scholar]
  9. Aries R. E., Gutteridge C. S., Ottley T. W. Evaluation of a low-cost, automated pyrolysis-mass spectrometer. J Anal Appl Pyrolysis 1986; 9:81–98
    [Google Scholar]
  10. Ludlam H. A., Noble W. C., Marples R. R., Phillips I. The evaluation of a typing scheme for coagulase-negative staphylococci suitable for epidemiological studies. J Med Microbiol 1989; 30:161–165
    [Google Scholar]
  11. Magee J. T., Hindmarch J. M., Bennett K. W., Duerden B. I., Aries R. E. A pyrolysis mass spectrometry study of fusobacteria. J Med Microbiol 1989; 28:227–236
    [Google Scholar]
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