Oxygen uptake and HO accumulation during the metabolism of glucose and glycerol by whole cells, and of L-α-glycerophosphate (GP) and NADH by cells lysed with Triton, was determined for the type strains of six fermentative species. Oxidation of glucose and of NADH by and was accompanied by the accumulation of relatively small quantities of HO (<0.05 mol/mol O), though larger quantities (0.17–0.24 mol/mol O) were produced by and were distinguished from the other strains used in that O uptake in the presence of glucose could not be demonstrated. However, metabolism of glucose was indicated by a reduction in the pH of the suspending medium and lysed cells oxidised NADH with the production of approximately 1.0 mol HO/mol O taken up. Glycerol was oxidised by all the strains studied except , and large quantities of HO (0.48–1.07 mol/mol O) accumulated. Cells of the glycerol-oxidising strains, lysed with Triton, oxidised GP with the production of approximately 1.0 mol HO/mol O utilised, which indicated the presence of a GP oxidase. The importance of HO production as a factor in the pathogenicity of some mycoplasmas might depend upon the availability of glycerol


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