1887

Abstract

Summary

The polymerase chain reaction was used to detect HSV-1 DNA in genomic DNA extracted from peripheral blood leucocytes, in patients with Behcet’s syndrome (BS), patients with recurrent oral ulcers and normal healthy controls. A 211-bp HSV-1 DNA fragment was found in a significant number of patients with BS (p <0.02). Serum anti-HSV-1 antibodies were also found in a higher proportion of patients with BS (p <0.02) than in healthy controls. However, virus-specific DNA was not detected in biopsy samples taken from oral ulcers in patients with BS.

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/content/journal/jmm/10.1099/00222615-34-1-39
1991-01-01
2022-08-19
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References

  1. Lehner T, Batchelor J. R. Classification and an immunogenetic basis of Behçet’s syndrome. In Lehner T, Barnes C. G. (eds) Behçet’s syndrome London: Academic Press; 197913–32
    [Google Scholar]
  2. Hooks J. J. Possibility of a viral etiology in recurrent aphthous ulcers and Behçefs syndrome. J Oral Pathol 1978; 7:353–364
    [Google Scholar]
  3. Behçet H. Über rezidiviemde, aphthose durch ein Virus verursachte Geschwiire am Mund, am Auge und and en Genitalien. Dermat Wchrschr 1937; 105:1152–1157
    [Google Scholar]
  4. Lehner T, Sagebiel R. W. Fine structural findings in recurrent oral ulceration. Brit Dent J 1966; 121:454–456
    [Google Scholar]
  5. Lehner T. Pathology of recurrent oral ulceration and oral ulceration in Behçets syndrome: Light, electron and fluorescence microscopy. J Pathol 1969; 97:481–494
    [Google Scholar]
  6. Sezer N. Further investigations on the virus of Behçet’s disease. Am J Ophthalmol 1956; 41:41–55
    [Google Scholar]
  7. Evans A. D, Pallis C. A., Spillane J. D. Involvement of the nervous system in Beliefs syndrome. Lancet 1957; 2:349–353
    [Google Scholar]
  8. Mortada A, Imam I. Z. E. Virus aetiology of Behçet’s syndrome. Brit J Ophthal 1964; 48:250–259
    [Google Scholar]
  9. Dowling G. B. Discussion on Behçet’s disease. Proc R Soc Med 1961; 54:101–104
    [Google Scholar]
  10. Denman A. M, Fialkow P. J., Pelton B. K, Salo A. C., Appleford D. J. Attempts to establish a viral aetiology for Behçet’s syndrome. In Lehner T, Barnes C. G. (eds) Behçet’s syndrome London: Academic Press; 197991–105
    [Google Scholar]
  11. Poulter L. W, Lehner T. Immunohistology of oral lesions from patients with recurrent oral ulcers and Behçet’s syndrome. Clin Exp Immunol 1989; 78:189–195
    [Google Scholar]
  12. Denman A. M., Fialkow P. J., Pelton B. K., Salo A. C., Appleford D. J., Gilchrist C. Lymphocyte abnormalities in Behçet’s syndrome. Clin Exp Immunol 1980; 42:175–185
    [Google Scholar]
  13. Hollingworth P, Hylton W., Pelton B. K., Denman A. M. Effects of immunosuppression on herpes simplex virus growth in lymphocytes of patients with connective tissue diseases. Ann Rheum Dis 1983; 42:264–69
    [Google Scholar]
  14. Bacon T. H., Özbakir F, Elms C. A., Denman A. M. Interferon-γ production by peripheral blood mononuclear cells from patients with Behçet’s syndrome. Clin Exp Immunol 1984; 57:541–547
    [Google Scholar]
  15. Young C, Lehner T., Barnes C. G. CD4 and CD8 cell responses to herpes simplex virus in Behçet’s disease. Clin Exp Immunol 1988; 73:6–10
    [Google Scholar]
  16. Hussain L, Ward R. G., Barnes C. G., Lehner T. Antibodies to herpes simplex virus in polyethylene glycol precipitable complexes and in sera from patients with Behçet’s disease. In Lehner T, Barnes C. G. (eds) Recent advances in Behçet’s disease International Congress and Symposium Series Royal Society of Medicine; 1986; 10373–77
    [Google Scholar]
  17. Eglin R. P., Lehner T., Subak-Sharpe J. H. Detection of RNA complimentary to herpes-simplex virus in mononuclear cells from patients with Behçet’s syndrome and recurrent oral ulcers. Lancet 1982; 2:1356–1361
    [Google Scholar]
  18. Bonass W. A., Bird-Stewart J. A, Chamberlain M. A., Halliburton I. W. Molecular studies in Behçet’s syndrome. In Lehner T, Barnes C. G. (eds) Recent advances in Behçet’s disease International Congress and Symposium Series Royal Society of Medicine; 1986; 10337–41
    [Google Scholar]
  19. Lehner T. Recurrent aphthous ulceration and autoimmunity. Lancet 1964; 2:1154–1155
    [Google Scholar]
  20. McGeoch D. J., Dolan A., Frame M. C. DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes. Nucl Acids Res 1986; 14:3435–3448
    [Google Scholar]
  21. Saiki R. K., Scharf S., Faloona F. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985; 230:1350–1354
    [Google Scholar]
  22. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1984; 137:266–267
    [Google Scholar]
  23. Rock D. L., Fraser N. W. Detection of HSV-1 genome in central nervous system of latently infected mice. Nature 1983; 302:523–525
    [Google Scholar]
  24. Wigdahl B, Scheck A. C., Ziegler R. J., De Clercq E, Rapp F. Analysis of the herpes simplex virus genome during in vitro latency in human diploid fibroblasts and rat sensory neurons. J Virol 1984; 49:205–213
    [Google Scholar]
  25. Efstathiou S, Minson A. C., Field H. J., Anderson J. R., Wildy P. Detection of herpes simplex virus-specific DNA sequences in latently infected mice and humans. J Virol 1986; 57:446–455
    [Google Scholar]
  26. Green M. T, Courtney R. J., Dunkel E. C. Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals. Infect Immunol 1981; 34:987–992
    [Google Scholar]
  27. Spivack J. G., Fraser N. W. Detection of herpes simplex virus type 1 transcripts during latent infection in mice. J Virol 1987; 61:3841–3847
    [Google Scholar]
  28. Rock D. L., Nesbum A. B., Ghiasi H. Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1. J Virol 1987; 61:3820–3826
    [Google Scholar]
  29. Deatly A. M., Spivack J. G., Lavi E., Fraser N. W. RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice. Proc Natl Acad Sci USA 1987; 84:3204–3208
    [Google Scholar]
  30. Honess R. W., Roizman B. Regulation of herpesvirus macromolecular synthesis. 1: Cascade regulation of the synthesis of three groups of viral proteins. J Virol 1974; 14:8–19
    [Google Scholar]
  31. Lehner T. The role of a disorder in immunoregulation, associated with herpes simplex virus type 1 in Behçet’s disease. In Lehner T., Barnes CG. (eds) Recent advances in Behçet’s disease Royal Society of Medicine, International Congress and Symposium Series; 1986; 10331–36
    [Google Scholar]
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