1887

Abstract

The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of an easily and rapidly prepared soluble protein fraction were used in conjunction with conventional techniques to identify different strains of and . Complete concordance of results from both methods was obtained with all species except those of the complex. With the SDS-PAGE technique, all strains of the complex were recognised as belonging to one species. By visual analysis of the SDS-PAGE polypeptide profiles, only minor differences between strains of the same species were seen and each species showed a characteristic polypeptide profile. Quantitation of the data by calculation of the Dice coefficient of similarity of the band positions obtained by densitometry indicated that the similarity between different strains of one species was 90–100% and the similarity between the species was in the range 30–45%. The results indicate that SDS-PAGE is a simple and rapid method for identifying mycobacterial strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-34-1-1
1991-01-01
2020-11-30
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-34-1-1
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error