Hepatotoxic factor(s) were isolated from whole-cell lysates of GIFU 8734 and purified by chromatography. A single intravenous injection of 10 µ of this factor reproducibly produced hepatitis in mice, as determined by histology and liver function tests. The hepatic lesions were very similar to those evoked by infection. Tissue-culture studies with mouse hepatocytes demonstrated that low concentrations of the factor caused release of hepatic enzymes into the medium without appreciable cytolysis. High concentrations of the factor induced cytolysis. These effects were neutralised by antiserum to the factor, but not by antisera to the lipopolysaccharide of or to the heat-labile enterotoxin of . Among 20 clinical isolates of , only four evoked hepatitis in mice and produced the hepatotoxic factor.


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