Ultrastructure of a spiral micro-organism from pig gastric mucosa () Free

Abstract

Summary

The ultrastructural features of a helical-shaped bacterium occurring inthe stomach of pigs, within the mucus on the mucosal surface of antral pits,were examined. The bacterial cell had three to eight spiral turns, flattened and truncated ends and was approximately 4.0 μm long and 0.6 μm wide.In some sections, up to six flagella, about 22 nm in diameter, were seen arising from each pole. The cytoplasm contained sparse, irregular granules,numerous ribosomes and large single-layered membrane-bound granules. In the flagella insertion area, there was a highly electron-dense component,the “polar membrane”. This micro-organism differed from similar bacteria described in cats, dogs and monkeys, and may cause inflammation in the antral mucosa of pigs similar to infection in man. Furthermore, it was morphologically similar to the spiral micro-organism distinct from which has been described recently in human antral mucosa from patients with gastritis and may be of potential significanceas a pathogen in man. The name is proposedfor this bacterium.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-33-1-61
1990-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/33/1/medmicro-33-1-61.html?itemId=/content/journal/jmm/10.1099/00222615-33-1-61&mimeType=html&fmt=ahah

References

  1. Kasai K, Kobayashi R. The stomach spirochaete occurring in mammals. J Parasitol 1919; 6:1–11
    [Google Scholar]
  2. Weber A F, Hasa O, Sauther J H. Some observations concerning the presence of spirilla in the fundic glands of dogs and cats. Am J Vet Res 1958; 19:677–680
    [Google Scholar]
  3. Warren J R, Marshall B J. Unidentified curved bacilli on gastric epithelium in active chronic gastritis ulceration. Lancet 1983; 1:1273–1274
    [Google Scholar]
  4. Curry A, Jones D M, Eldridge J. Spiral organisms in the baboon stomach. Lancet 1987; 2:634–635
    [Google Scholar]
  5. Lee A, Hazell S L, O’Rourke J, Kouprach S. Isolation of a spiral-shaped bacterium from the cat stomach. Infect lmmun 1988; 56:2843–2850
    [Google Scholar]
  6. Sato T, Takeuchi A. Infection by spirilla in the stomach of the rhesus monkey. Vet Pathol 1982; 19:Suppl:17–25
    [Google Scholar]
  7. Graham D Y. Campylobacter pylori and peptic ulcer disease. Gastroenterol 1989; 96:615–625
    [Google Scholar]
  8. Queiroz D M M, Rocha G A, Mendes E N, Lage A P, Carvalho ACT, Barbosa A J A. Spiral micro-organism in stomach of pigs. Vet Microbiol (in press)
    [Google Scholar]
  9. Dent J C, McNulty CAM, Uff JC, Wilkinson S P, Gear M W T. Spiral organisms in the gastric antrum. Lancet 1987; 2:96
    [Google Scholar]
  10. McNulty CAM, Dent J C, Curry A. New spiral bacterium in gastric mucosa. J Clin Pathol 1989; 42:585–591
    [Google Scholar]
  11. Rocha G A, Queiroz D M M, Mendes E N, Lage A P, Barbosa A J A. Simple carbol fuchsin staining for showing C. pylori and other spiral bacteria in gastric mucosa. J Clin Pathol 1989; 42:1004–1005
    [Google Scholar]
  12. McNulty C A M, Wise R. Rapid diagnosis of Campylobacter-associated gastritis. Lancet 1985; 1:1443–1444
    [Google Scholar]
  13. Coiro J R R, Weigl D R, Kiselins J, Menezes H, Billota J A T. A new embedding medium (Polylite 8001) for biological material. Cien Cult 1972; 24:660–662
    [Google Scholar]
  14. Lee A, Hazell S L. Campylobacter pylori in health and disease: an ecological perspective. Microb Ecol Health Dis 1988; 1:1–16
    [Google Scholar]
  15. Newell D G, Hudson M J, Baskerville A. Naturally occurring gastritis associated with Campylobacter pylori infection in the rhesus monkey. Lancet 1987; 2:1338
    [Google Scholar]
  16. Krakowka S, Morgan D R, Kraft W G, Leunk R D. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun 1987; 55:2789–2796
    [Google Scholar]
  17. Lambert J R, Borromeo M, Pinkard K J, Turner H, Chapman C B, Smith M L. Colonization of gnotobiotic piglets with Campylobacterpyloridis—an animal model?. J Infect Dis 1987; 155:1344
    [Google Scholar]
  18. Jorgensen A, Egnstrand L, Soderlind O, Gustafsson S. Pigs as a model for Campylobacter pylori infection. Abstracts of the 10th International Congress of the Pig Veterinary Society 1988131
    [Google Scholar]
  19. Vaira D, D’Anastasia C, Holton J. Campylobacter pylori in abattoir workers: is it a zoonosis?. Lancet 1988; 2:725726
    [Google Scholar]
  20. Murray R G E, Birch-Andersen A. Specialised structure in the region of the flagella tuft in Spirillum serpens. Can J Microbiol 1963; 9:393–402
    [Google Scholar]
  21. Krieg N R. Biology of the chemoheterotrophic spirilla. Bacteriol Rev 1976; 40:55–115
    [Google Scholar]
  22. Murray R G E. On the cell wall structure of Spirillum serpens. Can J Microbiol 1963; 9:381–392
    [Google Scholar]
  23. Lockard V G, Boler R K. Ultrastructure of a spiraled micro organism in the gastric mucosa of dogs. Am J Vet Res 1970; 31:1453–1462
    [Google Scholar]
  24. McCoy E C, Doyle D, Wiltberger H, Burda K, Winter A J. Flagellar ultrastructure and flagella-associated antigens of Campylobacter fetus. J Bacteriol 1975; 122:307–315
    [Google Scholar]
  25. Goodwin C S, McCulloch R K, Armstrong J A, Wee S H. Unusual cellular fatty acids and distinctive ultrastructure in a new spiral bacterium (Campylobacter pylori) from the human gastric mucosa. J Med Microbiol 1985; 19:257–267
    [Google Scholar]
  26. Krieg N R. Aerobic/microaerophilic, motile, helical/vibrioid Gram-negative bacteria. In: Krieg N R, Holt J G. (eds) Bergey’s manual of systematic bacteriology, 1st edn.. Baltimore, MD: Williams & Wilkins Co.; 198471–124
    [Google Scholar]
  27. Shively J M. Inclusion bodies of prokaryotes. Annu Rev Microbiol 1974; 28:167–187
    [Google Scholar]
  28. Lapage S P, Sneath P H A, Lessel E F, Skerman V B D, Seeliger H P R, Clark W A. (eds) International code of nomenclature of bacteria. Washington DC: American Society for Microbiology; 1975
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-33-1-61
Loading
/content/journal/jmm/10.1099/00222615-33-1-61
Loading

Data & Media loading...

Most cited Most Cited RSS feed