1887

Abstract

Summary

Fresh clinical isolates of spp. and were analysed by pyrolysis-mass spectrometry (Py-MS). The results formed the basis of mathematically derived characterisations of individual strains and these were compared with the results of phage typing for the salmonellas and M protein typing for the streptococci. Py-MS was shown to be a rapid and reproducible method for inter-strain comparisons, giving evidence of identity and non-identity between strains that agreed well with the results of conventional tests. Py-MS has potential value as a rapid, relatively inexpensive and highly discriminatory method of epidemiological analysis in bacterial disease.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-4-283
1990-08-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/4/medmicro-32-4-283.html?itemId=/content/journal/jmm/10.1099/00222615-32-4-283&mimeType=html&fmt=ahah

References

  1. Gutteridge CS, Norris JR. The application of pyrolysis techniques to the identification of micro-organisms. J Appl Bacterial 1979; 47:5–43
    [Google Scholar]
  2. Shute LA, Berkeley RCW, Norris JR, Gutteridge CS. Pyrolysis mass-spectrometry in bacterial systematics. In: Goodfellow M, Minnikin DE. (eds) Chemical methods in bacterial systematics London: Academic Press; 198595–114
    [Google Scholar]
  3. Eshuis W, Kistemaker PG, Meuzelaar HLC. Some numer ical aspects of reproducibility and specificity. In: Jones ER, Cramers CA. (eds) Analytical pyrolysis Amsterdam: Elsevier; 1977151–166
    [Google Scholar]
  4. Meuzelaar HL, Haverkamp J. Pyrolysis mass spectrometry of recent and fossil biomaterials. (Techniques and instrumentation in analytical chemistry 3) 1982 Elsevier; Amsterdam:
    [Google Scholar]
  5. Gutteridge CS, Vallis L, MacFie HJH. Numerical methods in the classification of micro-organisms by pyrolysis mass-spectrometry. In: Goodfellow M, Jones D, Priest FG. (eds) Computer-assisted bacterial systematics London: Academic Press; 1985369–401
    [Google Scholar]
  6. Shute LA, Gutteridge CS, Berkeley RCW. Pyrolysis mass spectrometry: a discriminatory technique with potential for rapid characterisation and identification of micro-organisms. XIV International Congress of Microbiology 1986 Manchester; Abstract
    [Google Scholar]
  7. Magee JT, Hindmarch JM, Duerden BI, MacKenzie DWR. Pyrolysis mass spectrometry as a method for interstrain discrimination of Candida albicans. J Gen Microbiol 1988; 134:2841–2847
    [Google Scholar]
  8. Aries RE, Gutteridge CS, Ottley TW. Evaluation of a low-cost, automated pyrolysis mass-spectrometer. J Anal Appl Pyrolysis 1986; 9:81–98
    [Google Scholar]
  9. Threlfall EJ, Rowe B, Ward LR. Subdivision of Salmonella enteritidis phage types by plasmid profile typing. Epidemiol Infect 1989; 102:459–465
    [Google Scholar]
  10. Magee JT, Hindmarch JM, Bennett KW, Duerden BI, Aries RE. A pyrolysis mass spectrometry study of fusobacteria. J Med Microbiol 1989; 28:227–236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-4-283
Loading
/content/journal/jmm/10.1099/00222615-32-4-283
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error