1887

Abstract

The effect of various antigens of promastigotes on the function of human polymorphonuclear leucocytes (PMNLs) was examined. Different concentrations of antigens were incubated with isolated PMNLs for various periods and the respiratory burst was assessed by Luminol-dependent chemilumi-nescence. All the antigens employed inhibited the PMNL respiratory burst by 35-64%. PMNL viability was not affected either by the concentrations or type of the parasite antigen. Oxygen free radical scavengers enhanced the action of the antigens on the PMNL respiratory burst.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-3-189
1990-07-01
2024-06-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/3/medmicro-32-3-189.html?itemId=/content/journal/jmm/10.1099/00222615-32-3-189&mimeType=html&fmt=ahah

References

  1. Al Gindan Y., Abdul Aziz O., Kubba R. Cutaneous leishmaniasis in Al-Hassa, Saudi Arabia. Int JDermatol 1984; 23:194–197
    [Google Scholar]
  2. Hussein M F. Leishmaniasis diseases in Saudi Arabia. Arab Biological Union: a Seminar on Leishmaniasis in Arabic Countries ; Amman, Jordan. 15-17 June; 1981
    [Google Scholar]
  3. Mahmoud A A., Al-Tuwaijri A S., Al-Mofleh I A., Al-Khuwaitir S A. In vitro and in vivo isolation of Leishmania tropica from Saudi Arabia. Zeit Parasitenk 1985; 71:271–272
    [Google Scholar]
  4. Al-Mofleh I., Al-Tuwaijri A., Mahmoud A. Effect of Leishmania major on Luminol-dependent chemiluminescence of whole blood phagocytosis. Trop Med Parasitol In press
    [Google Scholar]
  5. Babior B M. Oxygen-dependent microbial killing by phagocytes. New Eng J Med 1978; 298:659–668
    [Google Scholar]
  6. Klebanof S J. Oxygen-dependent cytotoxic mechanisms of phagocytes. In Gallin J I., Fauci A S. eds Advances in host defense mechanisms. vol 1 New York: Raven Press; 1982111–162
    [Google Scholar]
  7. Lowry O H., Rosebrough N J., Farr A L., Randall R J. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193:265–275
    [Google Scholar]
  8. Tono-Oka T., Ueno N., Matsumoto T., Ohkawa M., Matsu-moto S. Chemiluminescence of whole blood. I. A simple and rapid method for the estimation of phagocytic function of granulocytes and opsonic activity in whole blood. Clin ImmunolImmunopathol 1983; 26:66–75
    [Google Scholar]
  9. Al-Mofleh I A. A macrophage immunosuppression induced by Leishmania major in BALB/c mice. Trop Med Parasitol 1987; 38:93–96
    [Google Scholar]
  10. Suthipark U., Krungkrai J., Jeampipatkul A., Yuthavong Y., Panijpan B. Superoxide dismutase (SOD) in mouse red blood cells infected with Plasmodium berghei . J Parasitol 1982; 68:337–339
    [Google Scholar]
  11. Arias A E., Walter R D. Plasmodium falciparum: association with erythrocytic superoxide dismutase. J Protozool 1988; 35:348–351
    [Google Scholar]
  12. Niedel J E., Kuhn L J., Vandenbark G R. Phorbol diester receptor copurifies with protein kinase C. Proc Natl Acad Sci USA 1983; 80:36–40
    [Google Scholar]
  13. Chung T., Kim Y B. Two distinct cytolytic mechanisms of macrophages and monocytes activated by phorbol myristate acetate. J Leukoc Biol 1988; 44:329–336
    [Google Scholar]
  14. Roos D., Bot A A M., Van Schaik M L J., De Beer M., Daha M R. Interaction between human neutrophils and zymosan particles: the role of opsonins and divalent cations. J Immunol 1981; 126:433–440
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-3-189
Loading
/content/journal/jmm/10.1099/00222615-32-3-189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error