RT Journal Article SR Electronic(1) A1 Pruul, H. A1 Goodwin, C. S. A1 Mcdonald, P. J. A1 Lewis, G. A1 Pankhurst, Dagmar.YR 1990 T1 Hydrophobic Characterisation of Helicobacter (Campylobacter) Pylori JF Journal of Medical Microbiology, VO 32 IS 2 SP 93 OP 100 DO https://doi.org/10.1099/00222615-32-2-93 PB Microbiology Society, SN 1473-5644, AB Summary Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chomato-graphy. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) of hydrophobicity in the two-phase system was determined for H. pylori. PC was dependent on pH and the PC value was increased by greater than 20-fold at pH 2.5. Lithium cations increased PC, indicating a net negative surface charge. The presence of urea prevented the relative loss of hydrophobicity at raised pH. Exposure of H. pylori to proteolytic enzymes reduced the ability of the bacteria to adhere to human polymorphonuclear neutrophils (PMN). These findings suggest that H. pylori possesses protein-associated hydrophobic factors that are responsible for the nonopsonic adherence to PMN cell membranes., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-32-2-93