1887

Abstract

Summary

A micro-enzyme linked immunosorbent assay (micro-ELISA) has been evaluated as a diagnostic test to detect amoebic antigen in polyethylene glycol (PEG) precipitated circulating immune complexes (CIC) in sera from patients with amoebiasis. The immune complexes were captured on rabbit anti-amoebic IgG-coated wells of microtitration plates and the complexed antigen was detected by enzyme linked antihuman immunoglobulins. A titre of > 160 for the immune complexes was considered to be of clinical significance. The immunoassay detected amoebic, antigen-specific CIC in 35 (94·5%) of 37 patients with confirmed amoebic liver abscess. Twenty (55·5%) of 36 clinically suspected cases of amoebic liver abscess had amoebic antigen-specific CIC and responded favourably to anti-amoebic chemotherapy. Only two (20%) of 10 cases of non-dysenteric symptomatic intestinal amoebic infection had amoebic antigen-specific CIC. One (10%) of 10 patients with non-amoebic intestinal disorders also had amoebic antigen in CIC. However, none of 15 cases of non-amoebic hepatic disorders that included hydatid disease, metastatic adenocarcinoma, hepatocellular carcinoma, cholecystitis and choledocal cyst, 13 cases of rheumatoid arthritis and 25 apparently healthy subjects had amoebic antigen in CIC. The levels of the amoebic antigen-specific CIC did not correlate (p>0·05) with either the number of abscess(es) or lobe(s) of the liver involved. However, the levels of antigen-specific CIC were higher (p<0·01) in patients with a liver size of more than 5 cm below the right costal margin. Antigen-specific CIC levels tended to decline or disappear during 3-6 months following completion of therapy. In spite of the limitations for diagnosing symptomatic intestinal amoebic disease, the demonstration of specific CIC is recommended as an immuno-diagnostic procedure for patients with suspected amoebic liver abscess.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-2-87
1990-06-01
2024-07-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/2/medmicro-32-2-87.html?itemId=/content/journal/jmm/10.1099/00222615-32-2-87&mimeType=html&fmt=ahah

References

  1. Abioye A A., Lewis E A., McFarlane H. 1972; Clinical evaluation of serum immunoglobulins in amoebiasis. Immunology 23:937–946
    [Google Scholar]
  2. Bos H J., Van den Eijk A A. 1976; Enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of amoebiasis. In Sepulveda S., Diamond L S. eds Memorias de la Conferencia Intemacional Sobre Amibiasis. Instituto Mexicano del Seguro Social; Mexico: pp 721–727
    [Google Scholar]
  3. Gandhi B M., Irshad M., AcharyaS K., Tandon B N. 1988; Amebic liver abscess and circulating immune complexes of Entamoeba histolytica proteins. American Journal of Tropical Medicine and Hygiene 39:440–444
    [Google Scholar]
  4. Healy G R., Visvesvara G R., Kagan I G. 1974; Observations on the persistence of antibodies of E. histolytica. Archives of Investigative Medicine (Mex) 5 Suppl 2 495–500
    [Google Scholar]
  5. Houba V., Allison A C., Hendrickse R G., de Petries S., Edgington G M., Adeniyi A. 1970; Immune complexes in the nephrotic syndrome of Nigerian children. In Bonomo L., Turk J L. eds Immune complex disease. Carlo Erba Foundation; Milan: pp 23–28
    [Google Scholar]
  6. Hudson L., Hay F C. 1980; Practical immunology. , 2nd edn. Blackwell Scientific Publications; Oxford: p 226
    [Google Scholar]
  7. Juniper K., Worrel C L., Minshew M C., Roth L S., Cypert H., Lyoyd R E. 1972; Serologic diagnosis of amebiasis. American Journal of Tropical Medicine and Hygiene 21:157–168
    [Google Scholar]
  8. Mohimen A., Maitra T K., Jalan K N., Mehra S. 1989; A specific solid-phase assay for the detection of immune complexes containing Entamoeba histolytica antigens. Journal of Immunological Methods 117:39–44
    [Google Scholar]
  9. Natali P G., Cioli D. 1976; Immune complex nephritis in Schistosoma mansoni-infected mice. European Journal of Immunology 6:359–364
    [Google Scholar]
  10. Ngu J L., Blackett K. 1977; Complement and immunoconglutinin changes in the nephrotic syndrome of adult Africans. American Journal of Tropical Medicine and Hygiene 73:250–254
    [Google Scholar]
  11. Nuti M., D’Amelio R., Seminara R., Palmisano L., Aiuti F. 1982; Circulating immune complexes in amoebiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 76:567–568
    [Google Scholar]
  12. Pillai S., Mohimen A. 1982; A solid-phase sandwich radioimmunoassay for Entamoeba histolytica proteins and detection of circulating antigens in amoebiasis. Gastroenterology 83:1210–1216
    [Google Scholar]
  13. Shahin B., Papadopoulou Z L., Jenis E H. 1974; Congenital nephrotic syndrome associated with congenital toxoplasmosis. Journal of Pediatrics 85:366–370
    [Google Scholar]
  14. Thomas V., Sinniah B., Leng Y P. 1981; Assessment of the sensitivity, specificity and reproducibility in the indirect immunofluorescent technique for the diagnosis of amebiasis. American Journal of Tropical Medicine and Hygiene 30:57–62
    [Google Scholar]
  15. Vinayak V K. 1975; The specificity of indirect haemagglutination test in the diagnosis of amoebiasis. Indian Journal of Preventive and Social Medicine 6:271–276
    [Google Scholar]
  16. Vinayak V K., Jain P., Gupta B., Kaushik S P., Sawhney S. 1980; Cellular and humoral responses in amoebic patients. Tropical and Geographical Medicine 32:298–302
    [Google Scholar]
  17. Vinayak V K., Purnima S., Singh K., Venkatwswarlu K., Nain C K., Mehta S K. 1986; Specific circulating immune complexes in amoebic liver abscess. Journal of Clinical Microbiology 23:1088–1090
    [Google Scholar]
  18. Walsh J A. 1986; Problems in recognition and diagnosis of amebiasis: Estimation of global magnitude of morbidity and mortality. Review of Infectious Diseases 8:228–238
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-2-87
Loading
/content/journal/jmm/10.1099/00222615-32-2-87
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error