Characterisation of a Unique Ceftazidime-Hydrolysing β-Lactamase, TEM-E2 Free

Abstract

Summary

A strain of Klebsiella , originally isolated in Liverpool in 1982, has been found to produce a novel transferable β-lactamase, TEM-E2. This enzyme confers resistance to ceftazidime and focused as a doublet band with an iso-electric point (pI) of 5·3. The strain also produced the TEM-1 β-lactamase. Both TEM-1 and TEM-E2 β-lactamases were encoded by a transferable 103 kb plasmid; these two enzymes also had similar molecular weights, were inhibited by clavulanic acid, and hydrolysed ampicillin, carbenicillin and cephaloridine at similar rates. However, unlike the TEM-1 enzyme, the TEM-E2 β-lactamase hydrolysed ceftazidime and cefotaxime with similar efficiency, although it conferred much greater resistance to ceftazidime in the host strain. This is the earliest documented example of a TEM-like enzyme which confers transferable resistance to ceftazidime and related cephalosporins.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-2-131
1990-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/2/medmicro-32-2-131.html?itemId=/content/journal/jmm/10.1099/00222615-32-2-131&mimeType=html&fmt=ahah

References

  1. Harper P B. The in-vitro properties of ceftazidime. J Antimicrob Chemother 1981; 8: Suppl B 5–13
    [Google Scholar]
  2. Richmond M H. β-actamase stability of cefotaxime. J Antimicrob Chemother 1980; 6 Suppl A:13–17
    [Google Scholar]
  3. Philippon A., Labia R., Jacoby G. Extended-spectrum β-lactamases. Antimicrob Agents Chemother 1989; 33:1131–1136
    [Google Scholar]
  4. Sirot J., Chanal C., Petit A., Sirot D., Labia R., Gerbaud G. Klebsiella pneumoniae and other Enterobacteriaceae producing novel plasmid-mediated β-lactamases markedly active against third-generation cephalosporins: epidemiologic studies. Rev Infect Dis 1988; 10:850–859
    [Google Scholar]
  5. Sougakoff W., Goussard S., Gerbaud G., Courvalin P. Plasmid-mediated resistance to third-generation cephalosporins caused by point mutations in TEM-type penicillinase genes. Rev Infect Dis 1988; 10:879–884
    [Google Scholar]
  6. Chanal C M., Sirot D L., Labia R. et al. Comparative study of a novel plasmid mediated β-lactamase, CAZ-2, and the CTX-1 and CAZ-1 enzymes conferring resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother 1988; 32:1660–1665
    [Google Scholar]
  7. Spencer R C., Wheat P F., Winstanlay T G., Cox D M., Plested S J. Novel β-lactamase in a clinical isolate of Klebsiella pneumoniae conferring unusual resistance to β-lactam antibiotics. J Antimicrob Chemother 1987; 20:919–927
    [Google Scholar]
  8. Bachmann B J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 1972; 36:525–557
    [Google Scholar]
  9. Amyes S G B., Gould I M. Trimethoprim resistance plasmids in faecal bacteria. Ann Microbiol (Inst Past) 1984; 135B:177–186
    [Google Scholar]
  10. Takahashi S., Nagano Y. Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 1984; 20:608–613
    [Google Scholar]
  11. Simpson I N., Harper P B O., Callaghan C H. Principal β-lactamases responsible for resistance to β-lactam antibiotics in urinary tract infections. Antimicrob Agents Chemother 1980; 17:929–936
    [Google Scholar]
  12. Matthew M., Harris A M., Marshall M J., Ross G W. The use of analytical isoelectric focusing for detection and identification of β-lactamases. J Gen Microbiol 1975; 88:169–178
    [Google Scholar]
  13. Eliasson I., Kamme C. Characterization of the plasmid mediated β-lactamase in Branhamella catarrhalis, with special reference to substrate affinity. J Antimicrob Chemother 1985; 15:139–149
    [Google Scholar]
  14. Reid A J., Amyes S G B. Plasmid penicillin resistance in Vibrio cholerae: identification of new β-lactamase SAR- 1. Antimicrob Agents Chemother 1986; 30:245–247
    [Google Scholar]
  15. Sykes R B., Bonner D P., Bush K., Georgopapadakou N H., Wells J S. Monobactams—monocyclic β-lactam antibiotics produced by bacteria. J Antimicrob Chemother 1981; 8: Suppl E 1–16
    [Google Scholar]
  16. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J 1964; 91:222–233
    [Google Scholar]
  17. Payne D J., Marriott M S., Amyes S G B. Mutants of the TEM-1 β-lactamase conferring resistance to ceftazidime. J Antimicrob Chemother 1989; 24:103–110
    [Google Scholar]
  18. Payne D J., Marriott M S., Amyes S G B. TEM-E1: a novel β-lactamase conferring resistance to ceftazidime. FEMS Microbiol Lett 1989; 50:97–100
    [Google Scholar]
  19. Gutmann L., Kitzis M D., Billet-Klein D. et al. Plasmid-mediated β-lactamase (TEM-7) involved in resistance to ceftazidime and aztreonam. Rev Infect Dis 1988; 10:860–866
    [Google Scholar]
  20. Knothe H., Shah P., Krcmery V., Antal M., Mitsuhashi S. Transferable resistance to cefotaxime, cefoxitin, cefa- mandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection 1983; 11:315–317
    [Google Scholar]
  21. Kliebe C., Nies B A., Meyer J F., Tolxdorff-Neutzling R M., Wiedemann B. Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother 1985; 28:302–307
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-2-131
Loading
/content/journal/jmm/10.1099/00222615-32-2-131
Loading

Data & Media loading...

Most cited Most Cited RSS feed