RT Journal Article SR Electronic(1) A1 Payne, D. J. A1 Marriott, M. S. A1 Amyes, S. G. B.YR 1990 T1 Characterisation of a Unique Ceftazidime-Hydrolysing β-Lactamase, TEM-E2 JF Journal of Medical Microbiology, VO 32 IS 2 SP 131 OP 134 DO https://doi.org/10.1099/00222615-32-2-131 PB Microbiology Society, SN 1473-5644, AB Summary A strain of Klebsiella oxytoca, originally isolated in Liverpool in 1982, has been found to produce a novel transferable β-lactamase, TEM-E2. This enzyme confers resistance to ceftazidime and focused as a doublet band with an iso-electric point (pI) of 5·3. The strain also produced the TEM-1 β-lactamase. Both TEM-1 and TEM-E2 β-lactamases were encoded by a transferable 103 kb plasmid; these two enzymes also had similar molecular weights, were inhibited by clavulanic acid, and hydrolysed ampicillin, carbenicillin and cephaloridine at similar rates. However, unlike the TEM-1 enzyme, the TEM-E2 β-lactamase hydrolysed ceftazidime and cefotaxime with similar efficiency, although it conferred much greater resistance to ceftazidime in the host strain. This is the earliest documented example of a TEM-like enzyme which confers transferable resistance to ceftazidime and related cephalosporins., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-32-2-131