1887

Abstract

Summary

Variation in typing of clinically significant isolates of coagulase-negative staphylococci (CNS) was determined by five typing methods with 143 isolates obtained from 19 patients over periods from 2 days to 1 year. In only one case did all isolates give exactly the same typing pattern by all five tests. No single method, or simple combination, provided a ready means of confirming the relatedness of separate isolates. The most frequently useful tests were antibiotic susceptibility and extrachromosomal DNA banding patterns. However, the results of biotyping, serotyping and phage typing were also helpful in showing the relationship between different isolates from a given patient. In most cases a core pattern varying by the gain or loss of a small number of features, characterised a given patient's isolates. In two cases, apparently radical changes in the infecting organism were observed, and confirmed by restriction endonuclease analysis. Care should be taken when successive isolates of CNS show distinct typing differences in deciding their clinical relevance.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-2-115
1990-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/2/medmicro-32-2-115.html?itemId=/content/journal/jmm/10.1099/00222615-32-2-115&mimeType=html&fmt=ahah

References

  1. Wilson W R., Danielson G K., Giuliani E R., Geraci J E. Prosthetic valve endocarditis. Mayo Clin Proc 1982; 57:155–161
    [Google Scholar]
  2. Caputo G M., Archer G L., Calderwood S B., DiNubile M J., Karchmer A W. Native valve endocarditis due to coagulase-negative staphylococci. Clinical and microbiological features. Am J Med 1987; 83:619–625
    [Google Scholar]
  3. Archer G L. Coagulase-negative staphylococci in blood cultures: the clinician’s dilemma. Infect Control 1985; 6:477–478
    [Google Scholar]
  4. Kirchhoff L V., Sheagren J N. Epidemiology and clinical significance of blood cultures positive for coagulase- negative staphylococcus. Infect Control 1985; 6:479–486
    [Google Scholar]
  5. Etienne J., Brun Y., el Solh N. et al. Characterization of clinically significant isolates from patients with endocarditis. J Clin Microbiol 1988; 26:613–617
    [Google Scholar]
  6. Parisi J T., Lampson B C., Hoover D L., Khan J A. Comparison of epidemiologic markers for Staphylococcus epidermidis. J Clin Microbiol 1986; 24:56–60
    [Google Scholar]
  7. Christensen G D., Parisi J T., Bisno A L., Simpson W A., Beachey E H. Characterization of clinically significant strains of coagulase-negative staphylococci. J Clin Microbiol 1983; 18:258–269
    [Google Scholar]
  8. Parisi J T., Hecht D W. Plasmid profiles in epidemiologic studies of infections by Staphylococcus epidermidis. J Infect Dis 1980; 141:637–643
    [Google Scholar]
  9. Hartstein A I., Valvano M A., Morthland V H., Fuchs P C., Potter S A., Crosa J H. Antimicrobic susceptibility and plasmid profile analysis as identity tests for multiple blood isolates of coagulase-negative staphylococci. J Clin Microbiol 1987; 25:589–593
    [Google Scholar]
  10. Renaud F., Freney J., Etienne J. et al. Restriction endonu clease analysis of Staphylococcus epidermidis DNA may be a useful epidemiological marker. J Clin Microbiol 1988; 26:1729–1734
    [Google Scholar]
  11. Schleifer K H. Taxonomy of coagulase-negative staphylo cocci. In Mardh P A., Schleifer K H. eds Coagulase- negative staphylococci. Stockholm Almqvist and Wik- sell International; 198611–26
    [Google Scholar]
  12. Freney J., Brun Y., Bes M. et al. Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., two species from human clinical specimens. Int J Systematic Bacteriol 1988; 38:168–172
    [Google Scholar]
  13. Bés M., Brun Y., Fleurette J. Nouveaux bactériophages de Staphylococcus epidermidis: evaluation de leur interêt épidémiologique. Ann Microbiol (Paris) 1984; 135B:165–176
    [Google Scholar]
  14. Pillet J., Orta B. Species and serotypes in coagulage-negative staphylococci,. In Jeljaszewicz J. ed Staphylococci and staphylococcal infections, Zbl Bakt . Suppl 10: Stuttgart, Gustav Fischer Verlag; 1981147–152
    [Google Scholar]
  15. Barry A L., Thornsberry C. Susceptibility testing: diffusion test procedures. In Lennette H. et al. eds Manual of clinical microbiology, 4th edn. Washington, DC: American Society for Microbiology; 1985978–987
    [Google Scholar]
  16. Holmes D S., Quigley M. A. rapid boiling method for the preparation of bacterial plasmids. Annal Biochem 1981; 114:193–197
    [Google Scholar]
  17. Macrina F L., Kopecko D J., Jones K R., Ayers D J., McCowen S M. multiple multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978; 1:417–420
    [Google Scholar]
  18. Thakker-Varia S., Jenssen W D., Moon-McDermott L., Weinstein M P., Dubin D T. Molecular epidemiology of macrolides-lincosamides-streptogramin B resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother 1987; 31:735–743
    [Google Scholar]
  19. Bismuth R., Zilhao R., Courvalin P. Gene heterogeneity for tetracycline resistance in Staphylococcus spp. in press
    [Google Scholar]
  20. Khan S A., Novick R P. Complete nucleotide sequence of pT181 a tetracycline resistance from Staphylococcus aureus. Plasmid 1983; 10:251–259
    [Google Scholar]
  21. Maniatis T., Fritsch E F., Sambrook J. Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory. New York, Cold Spring Harbor; 1982382–389
    [Google Scholar]
  22. Mickelsen P A., Plorde J J., Gordon K P. et al. Instability of antibiotic resistance in a strain of Staphylococcus epidermidis isolated from an outbreak of prosthetic valve endocarditis. J Infect Dis 1985; 152:50–58
    [Google Scholar]
  23. Archer G L., Vishniavsky N., Stiver H B. Plasmid pattern analysis of staphylococcal epidermis isolates from patients with prosthetic valve endocarditis. Infect Immun 1982; 35:627–632
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-2-115
Loading
/content/journal/jmm/10.1099/00222615-32-2-115
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error