1887

Abstract

Summary

The adherence of clinical isolates of to cultured mouse epidermal cells was studied. Adherence of the isolates to the cells varied from strain to strain. When epidermal cell differentiation was induced by raising the calcium concentration in the medium, three out of 10 strains tested adhered better to calcium-induced differentiated cells than to undifferentiated cells, and one strain demonstrated higher adherence to undifferentiated cells than to differentiated cells. No significant difference between the adherences to both types of epidermal cells was observed with the other six strains. No relationship was observed between adherence and surface hydrophobicity with bacterial cells. Lipoteichoic acid and -acetyl sugars caused limited inhibition of adherence. The adherence assay method employed in this study is useful for investigating the effects of epidermal cell differentiation on bacterial adherence .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-32-1-9
1990-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/32/1/medmicro-32-1-9.html?itemId=/content/journal/jmm/10.1099/00222615-32-1-9&mimeType=html&fmt=ahah

References

  1. Aly R, Mai bach H I, Shinefield H R. 1977; Microbial flora of atopic dermatitis. Archives of Dermatology 113:780–782
    [Google Scholar]
  2. Aly R, Shinefield H R, Litz C, Maibach H I. 1980; Role of teichoic acid in the binding of Staphylococcus aureus to nasal epithelial cells. Journal of Infectious Diseases 141:463–465
    [Google Scholar]
  3. Aly R, Levit S. 1987; Adherence of Staphylococcus aureus to squamous epithelium: role of fibronectin and teichoic acid. Reviews of Infectious Diseases 9:S341–S350
    [Google Scholar]
  4. Beuth J, Ko H L, Ohshima Y, Yassin A, Uhlenbruck G, Pulverer G. 1988; The role of lectins and lipoteichoic acid in adherence of Staphylococcus saprophyticus. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene A 268:357–361
    [Google Scholar]
  5. Bibel D J, Aly R, Shinefield H R, Maibach H I, Strauss W G. 1982; Importance of the keratinized epithelial cell in bacterial adherence. Journal of Investigative Dermatology 79:250–253
    [Google Scholar]
  6. Carruthers M M, Kabat W J. 1983; Mediation of staphylococcal adherence to mucosal cells by lipoteichoic acid. Infection and Immunity 40:444–446
    [Google Scholar]
  7. Fletcher M, Loeb G I. 1979; Influence of substratum characteristics on the attachment of marine pseudomonad to solid surfaces. Applied and Environmental Microbiology 37:6772
    [Google Scholar]
  8. Fuquay J I, Loo D T, Barnes D W. 1986; Binding of Staphylococcus aureus by human serum spreading factor in an in vitro assay. Infection and Immunity 52:714–717
    [Google Scholar]
  9. Hanahan D J, Olley J N. 1958; Chemical nature of monophos- phoinositides. Journal of Biological Chemistry 231:813–828
    [Google Scholar]
  10. Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa S H. 1980; Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19:245–254
    [Google Scholar]
  11. Hennings H, Holbrook K A. 1983; Calcium regulation of cell-cell contact and differentiation of epidermal cells in culture— An ultrastructural study. Experimental Cell Research 143:127–142
    [Google Scholar]
  12. Holderbaum D, Hall G S, Ehrhart L A. 1986; Collagen binding to Staphylococcus aureus. Infection and Immunity 54:356–364
    [Google Scholar]
  13. Leyden J J, Marples R R, Kligman A M. 1974; Staphylococcus aureus in the lesions of atopic dermatitis. British Journal of Dermatology 90:525–530
    [Google Scholar]
  14. Lopes J D, dos Reis M, Bretani R R. 1985; Presence of laminin receptors in Staphylococcus aureus. Science 229:275–277
    [Google Scholar]
  15. Lowry O H, Roberts N R, Leiner K Y, Wu M-L, Farr A L. 1954; The quantitative histochemistry of brain. Journal of Biological Chemistry 207:1–17
    [Google Scholar]
  16. Maxe I, Ryden C, Wadstrom T, Rubin K. 1986; Specific attachment of Staphylococcus aureus to immobilized fibro-nectin. Infection and Immunity 54:695–704
    [Google Scholar]
  17. Minagi S, Miyake Y, Akagawa Y, Tsuru H, Suginaka H. 1986a; Effect of saliva on candidal adherence to polymethyl methacrylate resin. Hiroshima Journal of Medical Sciences 35:93–97
    [Google Scholar]
  18. Minagi S, Miyake Y, Fujioka Y, Tsuru H, Suginaka H. 1986b; Cell-surface hydrophobicity of Candida species as determined by the contact-angle and hydrocarbon-adherence methods. Journal of General Microbiology 132:1111–1115
    [Google Scholar]
  19. Miyake Y, Kohada A, Fujii I, Sugai M, Suginaka H. 1989; Aminoglycosides enhance the adherence of Staphylococcus aureus to HeLa cells. Journal of Antimicrobial Chemotherapy 23:79–86
    [Google Scholar]
  20. Nemanic M K, Whitehead J S, Elias P M. 1983; Alterations in membrane sugars during epidermal differentiation: visualization with lectins and role of glycosidases. Journal of Histochemistry and Cytochemistry 31:887–897
    [Google Scholar]
  21. Ray T L, Wuepper K D. 1976; Experimental cutaneous candidiasis in rodents. Journal of Investigative Dermatology 66:29–33
    [Google Scholar]
  22. Ray T L, Payne C D. 1988; Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase. Infection and Immunity 56:1942–1949
    [Google Scholar]
  23. Rosenberg M, Gutnick D, Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  24. Ryden C, Rubin K, Speziale P, Hook M, Lindberg M, Wadstrom T. 1983; Fibronectin receptors from Staphylococcus aureus. Journal of Biological Chemistry 258:33963401
    [Google Scholar]
  25. Sugai M, Enomoto T, Miyake Y, Suginaka H. 1987; Extracellular products of Staphylococcus aureus reversibly inhibit the terminal differentiation of cultured mouse epidermal cells. Cell Structure and Function 12:395–399
    [Google Scholar]
  26. Yuspa S H, Hawley-Nelson P, Stanley J R, Hennings H. 1980; Epidermal cell culture. Transplantation Proceedings 12:Suppl 1114–122
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-32-1-9
Loading
/content/journal/jmm/10.1099/00222615-32-1-9
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error