%0 Journal Article %A Nguyen, V. K. %A Rihn, B. %A Heckel, C. %A Bisseret, F. %A Girardot, R. %A Monteil, H. %T Enzyme immunoassay (ELISA) for detection of Clostridium difficile toxin B in specimens of faeces %D 1990 %J Journal of Medical Microbiology, %V 31 %N 4 %P 251-257 %@ 1473-5644 %R https://doi.org/10.1099/00222615-31-4-251 %I Microbiology Society, %X Summary. Antisera against Clostridium difficile toxin B were prepared in sheep and rabbit and were used in indirect and sandwich enzyme-linked immunosorbent assays (ELISA) for the detection of toxin B. Polyvinyl chloride and polystyrene microtitration plates were tested as solid phases for the assay. Both assays had a lower limit of detection for toxin B of 1 ng/ml. They were used to detect the presence of toxin B in 210 human faecal specimens and also in the culture supernatant fluids of C. difficile strains isolated from the faecal samples. There was a close correlation between the results of sandwich ELISA and those of cytotoxicity tests and isolation of C. difficile. Our sandwich ELISA method seems to be useful as a presumptive test for detection of C. difficile toxin B. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-31-4-251