1887

Abstract

Antisera against toxin B were prepared in sheep and rabbit and were used in indirect and sandwich enzyme-linked immunosorbent assays (ELISA) for the detection of toxin B. Polyvinyl chloride and polystyrene microtitration plates were tested as solid phases for the assay. Both assays had a lower limit of detection for toxin B of 1 ng/ml. They were used to detect the presence of toxin B in 210 human faecal specimens and also in the culture supernatant fluids of strains isolated from the faecal samples. There was a close correlation between the results of sandwich ELISA and those of cytotoxicity tests and isolation of . Our sandwich ELISA method seems to be useful as a presumptive test for detection of toxin B.

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1990-04-01
2024-03-28
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References

  1. Banno Y, Kobayashi T, Watanabe K, Ueno K, Nozawa Y. 1981; Two toxins (D-1 and D-2) of Clostridium difficile causing antibiotic-associated colitis: purification and some characterization. Biochemistry International 2:629–635
    [Google Scholar]
  2. Borriello S P, Honour P. 1981; Simplified procedure for the routine isolation of Clostridium dificile from faeces. Journal of Clinical Pathology 34:1124–11230
    [Google Scholar]
  3. Borriello S P, Honour P. 1983; Concomitance of cytotoxigenic and non-cytotoxigenic Clostridium difficile in stool specimens. Journal of Clinical Microbiology 18:1006–10010
    [Google Scholar]
  4. Bradford M M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Dowell V R, Hawkins T M. 1974; Laboratory methods in anaerobic bacteriology. CDC laboratory manual, Communicable Disease Center; Atlanta, GA: Available from US. Government Printing Office Washington, D.C 57
    [Google Scholar]
  6. Ehrich M, Van Tassell R L, Libby J M, Wilkins T D. 1980; Production of Clostridium dificile antitoxin. Infection and Immunity 28:1041–10420
    [Google Scholar]
  7. Garvey J S, Cremer N E, Sussdorf D H. 1977; Isolation of immunoglobulins, antibodies and their subunits. In : Methods in immunology, 3rd edn.. W A Benjamin, Reading, MA;215
    [Google Scholar]
  8. George W L, Sutter V L, Finegold S M. 1978; Toxigenicity and antimicrobial susceptibility of Clostridium difficile, a cause of antimicrobial agent-associated colitis. Current Microbiology 1:55–58
    [Google Scholar]
  9. George W L, Sutter V L, Citron D, Finegold S M. 1979; Selective and differential medium for isolation of Clostridium difficile. Journal of Clinical Microbiology 9:214–219
    [Google Scholar]
  10. Krishnan C. 1986; Detection of Clostridium difficile toxins by enzyme immunoassay. Journal of Hygiene 96:5–12
    [Google Scholar]
  11. Levine H G, Kennedy M, LaMont J T. 1982; Counterimmuno–electrophoresis vs. cytotoxicity assay for the detection of Clostridium dificile toxin. Journal of Infectious Diseases 145:398
    [Google Scholar]
  12. Lyerly D M, Phelps C J, Wilkins T D. 1985; Monoclonal and specific polyclonal antibodies for immunoassay of Clostridium difficile toxin . Journal of Clinical Microbiology 21:11–21
    [Google Scholar]
  13. Lyerly D M, Roberts M D, Phelps C J, Wilkins T D. 1986; Purification and properties of toxins A and B of Clostridium difficile. FEMS Microbiology Letters 33:31–35
    [Google Scholar]
  14. Place J D, Schroeder H R. 1982; The fixation of anti-HBsAg on plastic surfaces%. Journal of Immunological Methods 48%:82–512
    [Google Scholar]
  15. Rihn B, Bisseret F, Girardot R, Scheftel J M, Nguyen V K, Monteil H. 1988; Fast protein purification of Clostridium dificile cytotoxin%. Journal of Chromatography 428:284–084
    [Google Scholar]
  16. Rolfe R D, Finegold S M. 1979; Purification and characterization of Clostridium difficile toxin. Infection and Immunity 25:51–912
    [Google Scholar]
  17. Shahrabadi M S, Bryan L E, Gaffney D, Coderre S E, Gordon R, Pai C H. 1984 Latex agglutination test for detection of Clostridium difficile toxin in stool samples Journal of Clinical Microbiology 20:03–393
    [Google Scholar]
  18. Sullivan N M, Pellett S, Wilkins T D. 1982; Purification and characterization of toxins A and B of Clostridium difficile%. Infection and Immunity 35:1032–10430
    [Google Scholar]
  19. Taylor N S, Thorne G M, Bartlett J G. 1981; Comparison of two toxins produced by Clostridium drficile. Infection and Immunity 34:1036–10430
    [Google Scholar]
  20. Tytgat F. 1980; Fréquence d‘isolement de Clostridium difficile dans les selles de malades hospitalisés: sensibilité aux antibiotiques des souches isolées. Annales de Microbiologie (Paris) 131B:11–20
    [Google Scholar]
  21. Viscidi R, Laughon B E, Hanvanich M, Bartlett J G, Yolken R H. 1984; Improved enzyme immunoassays for the detection of antigens in faecal specimens. Investigation and correction of interfering factors. Journal of Immunological Methods 67:71–291
    [Google Scholar]
  22. Yolken R H et al. 1981 Enzyme immunoassay for the detection of Clostridium difficile antigen Journal of Infectious Diseases 144:378
    [Google Scholar]
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