1887

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Volume 31, Issue 4

Plasmid and chromosomal related toxin polymorphism of serogroup O138; plasmid transfer and co-integration with pRP4 Free

Abstract

. DNA-DNA hybridisation was used to examine 89 isolates of serogroup O138 for toxin gene content and location. Sixty-six isolates encoded toxin genes; 29 were STpa STpb VT2, 24 were VT2, four were STpa STpb, three wereSTpb VT2, three were STpb VT2 LT and one was STpa. None were K88-, K99- or F41-positive. The VT toxin gene was chromosomally located in all VT isolates tested whereas STpa, STpb and LT were plasmid borne. In co-transfer experiments with pRP4 as the mobilising plasmid, VT2 genes were not transferred to recipient K12 whereas STpa, STpb and LT genes were. Two VT2-positive isolates inhibited pRP4 transfer to K12 by a factor of 10 and rare transconjugants harboured a cointegrate plasmid comprising pRP4 (54kb) and a cryptic host plasmid (145 kb). In a K12 background, the co-integrate had transfer and incompatibility properties of pRP4 whereas in both progenitor O138 backgrounds the co-integrate was transfer inhibited and in one case resolved to give original plasmids.

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© J. MED. MICROBIOL. 1990
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1990-04-01
2023-03-20
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References

  1. Anderson D G, Moseley S L. 1988; Escherichia coli F41 adhesin: Genetic organisation, nucleotide sequence, and homology with the K88 determinant.. Journal of Bacteriology 170:4890–4896
     [Google Scholar]
  2. Arthur A, Sherratt D. 1979; Dissection of the transposition process: a transposon-encoded site-specific recombination system.. Molecular and General Genetics 175:267–274
     [Google Scholar]
  3. Bennett P M, Heritage J, Comanducci A, Dodd H M. 1986; Evolution of R plasmids by replicon fusion.. Journal of Antimicrobial Chemotherapy 18:Suppl C103–111
     [Google Scholar]
  4. Birnboim H C, Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Research 7:1513–1523
     [Google Scholar]
  5. Colson C, Glover S W, Symonds N, Stacey K A. 1965; The location of the genes for host-controlled modification and restriction in Escherichia coli K12.. Genetics 52:1043–1050
     [Google Scholar]
  6. Corpet D E. 1986; Ecological factors influencing the transfer of plasmids in citro and in cico.. Journal of Antimicrobial Chemotherapy 18:Suppl C127–132
     [Google Scholar]
  7. Dallas W S, Gill D M, Falkow S. 1979; Cistrons encoding Escherichia coli heat-labile toxin.. Journal of Bacteriology 139:850–858
     [Google Scholar]
  8. Dobrescu L. 1983; New biological effect of edema disease principle (Escherichia coli-neurotoxin) and its use as an incitro assay for this toxin.. American Journal of Veterinary Research 44:31–34
     [Google Scholar]
  9. Farrar W E. 1983; Molecular analysis of plasmids in epidemiologic investigation.. Journal of Infectious Diseases 148:1–6
     [Google Scholar]
  10. Feinberg A P, Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.. Analytical Biochemistry 132:6–13
     [Google Scholar]
  11. Gannon V P, Gyles C L. Friendship R W 1988 Characteristics of verotoxigenic Escherichia coli from pigs.. Canadian Journal of Veterinary Research 52:331–337
     [Google Scholar]
  12. de Graaf F K, Krenn B E, Klaasen P. 1984; Organisation and expression of genes involved in the biosynthesis of K99 fimbriae.. Infection and Immunity 43:508–514
     [Google Scholar]
  13. Karmali M A. 1989; Infection of Verocytotoxin-producing Escherichia coli.. Clinical Microbiology Reviews 2:15–38
     [Google Scholar]
  14. Kleckner N. 1981; Transposable elements in prokaryocytes.. Annual Reviews of Genetics 15:341–404
     [Google Scholar]
  15. Lathe R., Hirth P, DeWilde M, Harford N, Lecocq J P. 1980; Cell-free synthesis of enterotoxin of E.coli from a cloned gene.. Nature 284:473–474
     [Google Scholar]
  16. Law D. 1988; Virulence factors of enteropathogenic Escherichia coli.. Journal of Medical Microbiology 26%:1–10
     [Google Scholar]
  17. Lee C H, Moseley S L, Moon H W, Whipp S C, Gyles C L, So M. 1983; Characterisation of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin I1 producers.. Infection and Immunity 42:264–268
     [Google Scholar]
  18. Linggood M A, Thompson J M. 1987; Verotoxin production among porcine strains of Escherichia coli and its association with oedema disease.. Journal of Medical Microbiology 24:359–362
     [Google Scholar]
  19. Mainil J, Bex F, Couturier M, Kaeckenbeeck A. 1985; Hybridization on bovine enterotoxigenic Escherichia coli with two heat-stable enterotoxin gene probes.. American Journal of Veterinary Research 46:2582–2584
     [Google Scholar]
  20. Maniatis T, Fritsch E F, Sambrook J. 1982; Molecular cloning: a laboratory manual.. Cold Spring Harbor Laboratory; Cold Spring Harbor, New York:
     [Google Scholar]
  21. Marques L R M, Peiris J S M, Cryz S J, O’Brien A D. 1987; Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin 11.. FEMS Microbiology Letters 44:33–38
     [Google Scholar]
  22. Matthewson J J. et al. 1985; A newly recognised cause of travelers’ diarrhea: enteroadherent Escherichia coli.. Journal of Infectious Diseases 151:471–475
     [Google Scholar]
  23. Nielson N O. 1981 Edema disease. Leman A D. Diseases of swine. Iowa State University Press; Ames:478–490
     [Google Scholar]
  24. Moseley S L, Huq I, Alim A R M A, So M, Samadpour-Motalebi M, Falkow S. 1980; Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.. Journal of Infectious Diseases 142:892–898
     [Google Scholar]
  25. Moseley S L, Dougan G, Schneider R A, Moon H W. 1986; Cloning of chromosomal DNA encoding the F41 adhesin of enterotoxigenic Escherichia coli and genetic homology between adhesins F41 and K88.. Journalof Bacteriology 167:799–804
     [Google Scholar]
  26. Pugsley A P, Oudega B. 1987 Hardy K G. Plasmids: a practical approach.. IRL Press; Oxfor:105–161
     [Google Scholar]
  27. Smith H W, Green P, Parsell Z. 1983; Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs.. Journalof General Microbiology 129:3121–3137
     [Google Scholar]
  28. Smith H R, Scotland S M, Rowe B. 1985 Genetics of Escherichia coli virulence.. Sussman M. The virulence of Escherichia coli: reviews and methods. Society for General Microbiology special publication Academic Press; London:227–269
     [Google Scholar]
  29. Smith H R, Scotland S M. 1988; Vero cytotoxin-producing strains of Escherichia coli.. Journal of Medical Microbiology 26:77–85
     [Google Scholar]
  30. Smith H R. et al. 1988; Vero cytotoxin production and presence of VT genes in Escherichia coli strains of animal origin.. Journal of General Microbiology 134:829–834
     [Google Scholar]
  31. Sojka W J . 1965; Escherichia coli in domestic animals and poultry.. Common wealth Agricultural Bureaux; Farnham Royal, UK:
     [Google Scholar]
  32. Sojka W J, Erskine R G, Lloyd M K. 1957; Haemolytic Escherichia coli and “oedema disease” of pigs.. Veterinary Record 69:293–301
     [Google Scholar]
  33. Thompson R. 1986; R Plasmid transfer.. Journal of Antimicrobial Chemotherapy18 Suppl C13–23
     [Google Scholar]
  34. Willshaw G A, Smith H R, Scotland S M, Rowe B. 1985; Cloning of genes determining the production of vero cytotoxin by Escherichia coli.. Journal of General Microbiology 131:3047–3053
     [Google Scholar]
  35. Willshaw G A, Smith H R, Scotland S M, Field A M, Rowe B. 1987; Heterogeneity of Escherichia coli phages encoding vero cytotoxins: comparison of cloned sequences determining VTl and VT2 and development of specific gene probes.. Journal of General Microbiology 133:1309–1317
     [Google Scholar]
  36. Woodward M J, McLaren I, Wray C. 1989; a Genetic evidence for a chromosomally integrated multiresistance plasmid in Salmonella dublin.. Journal of Medical Microbiology 28:205–210
     [Google Scholar]
  37. Woodward M J, McLaren I, Wray C. 1989; b Distribution of virulence plasmids within salmonellae.. Journal of General Microbiology 135:503–511
     [Google Scholar]
  38. Woodward M J, Kearsley R, Wray C, Roeder P L. 1989c; DNA probes for the detection of toxin genes in Escherichia coli isolated from diarrhoea1 disease in cattle and pigs.. (In press.)
     [Google Scholar]
  39. Wray C, McLaren I, Parkinson N M, Beedell Y. 1987; Differentiation of Salmonella typhimurium DT204C by plasmid profile and biotype.. Veterinary Record 121:514–516
     [Google Scholar]
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Plasmid and chromosomal related toxin polymorphism of Escherichia coli serogroup O138; plasmid transfer and co-integration with pRP4
J. Med. Microbiol. 31, 241 (1990); https://doi.org/10.1099/00222615-31-4-241
/content/journal/jmm/10.1099/00222615-31-4-241
/content/journal/jmm/10.1099/00222615-31-4-241
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