1887

Abstract

Summary

A clinical strain of isolated in Nottinghamshire in 1980 was shown to harbour the type IIIa trimethoprim-resistant dihydrofolate reductase gene, previously identified on only one occasion, in New Zealand in 1979. The gene was identified by hybridisation with an 855-bp type III gene probe and its classification as a type IIIa dihydrofolate reductase was confirmed by detailed biochemical analysis of the enzyme product. The dihydrofolate reductase was identical in size and isoelectric point with the original type IIIa enzyme and shared similar inhibitory and kinetic profiles. The trimethoprim resistance gene was subsequently cloned and the type IIIa dihydrofolate reductase gene was localised to a 700-bp RI-I fragment. This smaller fragment may prove to be a more specific DNA probe for the future identification of type IIIa dihydrofolate reductase genes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-31-3-213
1990-03-01
2022-08-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/31/3/medmicro-31-3-213.html?itemId=/content/journal/jmm/10.1099/00222615-31-3-213&mimeType=html&fmt=ahah

References

  1. Fleming M P, Datta N, Gruneberg R N. Trimethoprim resistance determined by R factors. Br Med J 1972; 1:726–728
    [Google Scholar]
  2. Amyes S G B,, Smith J T. R-factor trimethoprim resistance mechanism : an insuceptible target site. Biochem Biophys Res Commun 1974; 58:412–418
    [Google Scholar]
  3. Amyes S G B. The success of plasmid-encoded resistance genes in clinical bacteria. J Med Microbiol 1989; 28:73–83
    [Google Scholar]
  4. Joyner S S, Fling M E, Stone D, Baccanari D P. Characterisation of an R-plasmid dihydrofolate reductase with a monomeric structure. J Biol Chem 1984; 259:5851–5856
    [Google Scholar]
  5. Young H-K, Amyes S G B. A new mechanism of plasmid trimethoprim resistance : characterisation of an inducible dihydrofolate reductase. J Biol Chem 1986; 261:2503–2505
    [Google Scholar]
  6. Fling M E, Walton L, Elwell L P. Monitoring of plasmidencoded, trimethoprim-resistant dihydrofolate reductase genes: detection of a new resistant enzyme. Antimicrob Agents Chemother 1982; 22:882–888
    [Google Scholar]
  7. Barg N, Hutson F, Amyes S G B, Thomson C J, Wharton M, Schaffner W. Two outbreaks of dysentery caused by antibiotic resistant Shigella sonnei (ARS) elaborating a type I11 dihydrofolate reductase (DHFR). Abstracts of the Annual Meeting of the American Society of Microbiology 1988c-108
    [Google Scholar]
  8. Young H-K, Jesudason M V, Koshi G, Amyes S G B. Unusual expression of new low-level trimethoprim resistance plasmids. J Clin Microbwll 1986; 24:61–64
    [Google Scholar]
  9. Towner K J, Venning B M, Pinn P A. Occurrence of transposable trimethoprim resistance in clinical isolates of Escherichia coli devoid of self-transmissi ble resistance plasmids. Antimicrob Agents Chemother 1982; 21:336–338
    [Google Scholar]
  10. Huovinen P. Trimethoprim resistance. Antimicrob Agents Chemother 1987; 31:1451–1456
    [Google Scholar]
  11. Carter G I, Towner K J, Slack R C B. Rapid detection of a specific trimethoprim resistance gene using a biotinylated DNA probe. J Antimicrob Chemother 1987; 20:335–341
    [Google Scholar]
  12. Fling M E, Richards C. The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7. Nucleic Acids Res 1983; 11:5147–5158
    [Google Scholar]
  13. Zolg J W, Hanggi U J, Zachau H G. Isolation of a small DNA fragment carrying the gene for dihydrofolate reductase from a trimethoprim resistance factor. MolGen Genet 197; 164:15–29
    [Google Scholar]
  14. Towner K J, Young H-K, Amyes S G B. Biotinylated DNA probes for trimethoprim-resistant dihydrofolate reductases types IV and V. J Antimicrob Chemother 1988; 22:285–291
    [Google Scholar]
  15. Maniatis T, Fritsch E F, Sambrook J. Molecular Cloning; A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbour Laboratory 1982
    [Google Scholar]
  16. Amyes S G B, Smith J T. The purification and properties of the trimethoprim-resistant dihydrofolate reductase mediated by the R-factor, R388. Eur J Biochem 1976; 61:597–603
    [Google Scholar]
  17. Broad D F, Smith J T. Classification of trimethoprim resistant dihydrofolate reductase mediated by Rplasmids using isoelectric focusing. Eur J Bwchem 1982; 125:617–622
    [Google Scholar]
  18. Fling M E, Kope J, Richards C. Characterisationofplasmid pAZl and the type III dihydrofolate reductase gene. Plasmid 1988; 19:30–38
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-31-3-213
Loading
/content/journal/jmm/10.1099/00222615-31-3-213
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error