1887

Abstract

Summary

Fifty-two strains of were examined for production of chloramphenicol acetyltransferase (CAT) by a rapid technique based upon induction of enzyme activity and chemical assay. This method was compared with one measuring the minimum inhibitory concentration (MIC) by agar dilution and a diffusion test with disks containing 10 μg, 30 μg and 50 μg of chloramphenicol. The MIC for 13 chloramphenicol-resistant strains was 16 mg/L and for 39 sensitive strains ≤4 mg/L. The chemical assay clearly distinguished resistant from sensitive strains; it was technically simple and provided results within 90 min. The distinction between sensitive and resistant bacteria in the disk diffusion assay was clearer with 10-μg than with 30-μg and 50-μg disks. However, the chemical CAT assay, with enzyme induction, is recommended when a rapid result is required.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-31-2-133
1990-02-01
2019-10-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-31-2-133
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error