1887

Abstract

Summary

The occurrence of various strains in the sputum of 15 patients with cystic fibrosis (CF) was monitored over periods ranging from 2 to 60 months. Isolates of were typed by four different techniques, namely serotyping, active and passive pyocin typing, and phage typing. The maximum number of different serotypes found in the patients was three (one serotype in nine patients; two serotypes in five patients; three serotypes in one patient). Pyocin and phage typing showed no marked differences between strains of the same serotype in individual patients. Exacerbations of chronic respiratory infection were not associated with changes in the sputum flora, the composition of strains in which remains constant over long periods in patients with CF.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-31-2-119
1990-02-01
2022-05-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/31/02/medmicro-31-2-119.html?itemId=/content/journal/jmm/10.1099/00222615-31-2-119&mimeType=html&fmt=ahah

References

  1. Asheshov E H. 1974; An assessment of the methods used for typing strains of P. aeruginosa. Arseni A. Proceedings of the 6th international congress of bacteriology. Leontiadi Medical Editions Athens:9–22
    [Google Scholar]
  2. Cystic fibrosis foundation 1983; Pulmonary infection in cystic fibrosis.. “GAP” Conference Report 7:4
    [Google Scholar]
  3. Diaz F, Mosovich L L, Neter E. 1970; Serogroups of Pseudomonas aeruginosa and the immune response of patients with cystic fibrosis.. Journal of Infectious Diseases 121:269–274
    [Google Scholar]
  4. Doudoroff M, Palleroni N J. 1974; Pseudomonas. Buchanan R E, Gibbons N E. Bergey's manual of determinative bacteriology, 8th edn. The Williams and Wilkins Company; Baltimore:217–243
    [Google Scholar]
  5. Govan J R W. 1978; Pyocin typing of Pseudomonas aeruginosa. Bergan T, Norris J R. Methods in microbiology, vol 10. Academic Press; New York:61–91
    [Google Scholar]
  6. Hancock R E W, Mutharia L M, Chan L, Darveau R P, Speert D P, Pier G B. 1983; Pseudomonas aeruginosa isolates from patients with cystic fibrosis : a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains.. Infection and Immunity 42:170–177
    [Google Scholar]
  7. Lanyi B, Bergan T. 1978; Serological characterization of Pseudomonas aeruginosa. Bergan T, Norris J R. Methods in microbiology, vol 10. Academic Press; New York:93–168
    [Google Scholar]
  8. Liu P V, Matsumoto H, Kusama H, Bergan T. 1983; Survey of heat-stable, major somatic antigens of Pseudomonas aeruginosa. International Journal of Systematic Bacteriology 33:256–264
    [Google Scholar]
  9. Ogle J W, Janda J M, Woods D E, Vasil M L. 1987; Characterization and use of a DNA probe as an epidemiological marker for Pseudomonas aeruginosa . Journal of Infectious Diseases 155:119–126
    [Google Scholar]
  10. Ojeniyi B, Baek L, Høiby N. 1985; Polyagglutinability due to loss of O-antigenic determinants in Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Acta Pathologica, Microbiologica et Immunologica Scandinavica Section B 93:7–13
    [Google Scholar]
  11. Osman M A M. 1965; Pyocin typing of Pseudomonas aeruginosa . Journal of Clinical Pathology 18:200–202
    [Google Scholar]
  12. Pitt T L, MacDougall J, Penketh A R L, Cooke E M. 1986; Polyagglutinating and non-typable strains of Pseudomonas aeruginosa in cystic fibrosis. Journal of Medical Microbiology 21:179–186
    [Google Scholar]
  13. Seale T W, Thirkill H, Tarpay M, Flux M, Rennert O M. 1979; Serotypes and antibiotic susceptibilities of Pseudomonas aeruginosa isolates from single sputa of cystic fibrosis patients. Journal of Clinical Microbiology 9:72–78
    [Google Scholar]
  14. Sheehan D J, Janda J M, Bottone E J . 1982; Pseudomonas aeruginosa: changes in antibiotic susceptibility, enzymatic activity, and antigenicity among colonial morphotypes. Journal of Clinical Microbiology 15:926–930
    [Google Scholar]
  15. Thomassen M J, Demko C A, Boxerbaum B, Stern R C, Kuchenbrod P J. 1979; Multiple isolates of Pseudomonas aeruginosa with differing antimicrobial susceptibility patterns from patients with cystic fibrosis. Journal of Infectious Diseases 140:873–880
    [Google Scholar]
  16. Williams R J, Govan J R W. 1973; Pyocine typing of mucoid strains of Pseudomonas aeruginosa isolated from children with cystic fibrosis. Journal of Medical Microbiology 6:409–412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-31-2-119
Loading
/content/journal/jmm/10.1099/00222615-31-2-119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error