Is resistance to novobiocin a reliable test for confirmation of the identification of ? Free

Abstract

Summary

, a coagulase-negative staphylococcus (CNS), causes acute urinary tract infection predominantly in young women (15-30 years). In the clinical microbiology laboratory identification and differentiation of from other CNS usually depends solely upon the demonstration of resistance to the antimicrobial agent novobiocin. Phenotypic characteristics of 36 novobiocin-resistant CNS isolated from the urine of patients with acute urinary tract infections were further analysed and the homogeneity of the isolates assessed. The organisms were speciated by the API STAPH identification system. Twenty-one isolates were (p > 97%), and there was one strain each of and (p > 97%). Of the remainder, three isolates were unidentifiable and a further nine had the characteristics associated with more than one species of CNS. Additional tests, including carbohydrate fermentation, antibiotic sensitivity and fluorogenic substrate utilisation, were performed on all isolates. Computer analysis of the results confirmed that testing for resistance to novobiocin selects a heterogeneous group of CNS composed of several different species.

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1989-12-01
2024-03-28
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References

  1. Bes M., Brun Y., Gayral J., Fleurette P., Laban P. 1985; Improvement of “API STAPH” gallery and identification of new species of staphylococci. In Jeljaszewicz J. (ed) The staphylococci. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene Suppl 14 Gustav Fischer Verlag; Stuttgart:
    [Google Scholar]
  2. Brun Y., Fleurette J., Forey F. 1978; Micromethod for biochemical identification of coagulase negative staphylococci. Journal of Clinical Microbiology 8:503–508
    [Google Scholar]
  3. Christensen G. D., Parisi J. T., Bisno A. L., Simpson W. A., Beachey E. H. 1983; Characterization of clinically significant strains of coagulase-negative staphylococci. Journal of Clinical Microbiology 18:258–269
    [Google Scholar]
  4. Hussain Z., Stoakes L., Stevens D. L., Schieven B. C., Lannigan R., Jones C. 1986; Comparison of the MicroScan system with the “API Staph-Ident” system for species identification of coagulase-negative staphylococci. Journal of Clinical Microbiology 23:126–128
    [Google Scholar]
  5. Kloos W. E., Schleifer K. H. 1975; Simplified scheme for routine identification of Staphylococcus species. Journal of Clinical Microbiology 1:82–87
    [Google Scholar]
  6. Lennox V. A., Ackerman V. P. 1984; Biochemical identification of bacteria by replicator methods on agar plates. Pathology 16:434–440
    [Google Scholar]
  7. Maskell R. 1974; Importance of coagulase-negative staphylococci as pathogens in the urinary tract. Lancet 1:1155–1158
    [Google Scholar]
  8. Morgan J. W. 1983; Abbreviated scheme for the presumptive identification of Staphylococcus saprophyticus from urine cultures. Journal of Clinical Microbiology 18:1272–1274
    [Google Scholar]
  9. Nachaie M. R. 1983; Aspects of the classification and identification of medically important staphylococci. PhD Thesis University of Newcastle-upon-Tyne;
    [Google Scholar]
  10. Sneath P. H. A. 1957; The application of computers to taxonomy. Journal of General Microbiology 17:201–226
    [Google Scholar]
  11. Torres Pereira A. 1962; Coagulase-negative strains of staphylococcus possessing antigen 51 as agents of urinary infection. Journal of Clinical Pathology 15:252–253
    [Google Scholar]
  12. Tselenis-Kotsowilis A. D., Koliomichalis M. P., Papavassiliou J. T. 1982; Acute pyelonephritis caused by Staphylococcus xylosus. Journal of Clinical Microbiology 16:593–594
    [Google Scholar]
  13. Yu P. K. U., Washington J. A. 1985; Identification of staphylococci. In Washington J. A. (ed) Laboratory procedures in clinical microbiology 2nd edn Springer Verlag; New York: pp 130–131
    [Google Scholar]
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