Selection of a vaccine strain of low residual virulence by chemical mutagenesis Free

Abstract

SUMMARY

Following incubation of the oral vaccine strain 2 with diethyl sulphate (DES), a mutant designated strain S105 was selected by screening surviving bacteria for reduced virulence for mice. Strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. Nevertheless, it was as effective as the parent strain in stimulating protective immunity to when given as an oral vaccine to sheep under both experimental and field conditions.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-30-2-143
1989-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/30/2/medmicro-30-2-143.html?itemId=/content/journal/jmm/10.1099/00222615-30-2-143&mimeType=html&fmt=ahah

References

  1. Alton G. G., Jones L. M., Pietz D. E. 1975; Laboratory techniques in brucellosis. 2nd edn World Health Organization Monorgraph series No. 55
    [Google Scholar]
  2. Auerbach C. 1967; The chemical production of mutations. The effect of chemical mutagens on cells and their genetic material is discussed. Science 158114–117
    [Google Scholar]
  3. Elberg S. S., Faunce K. 1957; Immunization against Brucella infection IV. Immunity conferred on goats by a non dependent mutant from a streptomycin-dependent mutant strain of Brucella melitensis. Journal of Bacteriology 73:211–217
    [Google Scholar]
  4. Elberg S. S., Meyer K. F. 1958; Caprine immunization against brucellosis. A summary of experiments on the isolation, properties and behaviour of a vaccine strain. Bulletin of the World Health Organization 19711–724
    [Google Scholar]
  5. Pelecanos M., Anderson T. 1964; The mutagenic activity of diethyl sulfate in Drosophila melanogaster 1. The dose-mutagenic response to larval and adult feeding. Mutation Research 1173–178
    [Google Scholar]
  6. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  7. Report 1986 Joint FAO/WHO Expert Committee on Brucellosis, Sixth Report World Health Organization Technical Report Series No. 740
    [Google Scholar]
  8. Xie Xin 1986; Orally administrable brucellosis vaccine: Brucella suis strain 2 vaccine. Vaccine 4212–216
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-30-2-143
Loading
/content/journal/jmm/10.1099/00222615-30-2-143
Loading

Data & Media loading...

Most cited Most Cited RSS feed