1887

Abstract

SUMMARY

An antigen was purified from H37Ra culture filtrate by immunoabsorbent affinity chromatography with CNBr-activated sepharose 4B column coupled with pooled γ-globulin fraction from patients with active tuberculosis. The column was washed extensively with PBS and eluted with 3M sodium thiocyanate. Peak fractions were pooled and used as coating antigen in an ELISA. Sera from 86 normal subjects and 54 patients with active tuberculosis were tested against the immunoabsorbed antigen by ELISA with biotin-conjugated anti-human globulin and avidin-peroxidase reagents. At a selected ‘cut-off’ dilution of 320, 49 (91%) of 54 sera from active cases and 8 (9.3%) of 86 sera from normal subjects gave positive test results—a sensitivity and specificity each of 91%, compared with our previous results of sensitivity 75% and specificity 83% with PPD as antigen.

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1989-10-01
2024-04-13
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References

  1. Affronti L. F., Taylor R., Hassan E. M. 1986; Comparison between tuberculo-proteins A and C in the serodiagnosis of tuberculosis by enzyme-linked immunosorbent assay. International Archives of Allergy and Applied Immunology 80:81–84
    [Google Scholar]
  2. Benjamin R. G., Debanne S. M., Ma Y., Daniel T. M. 1984; Evaluation of mycobacterial antigen in an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis. Journal of Medical Microbiology 18:309–318
    [Google Scholar]
  3. Chawla T. C., Sharma A., Kiran U., Bhargava D. K., Tandon B. N. 1986; Serodiagnosis of intestinal tuberculosis by enzyme immunoassay and soluble antigen fluorescent antibody tests using a saline extracted antigen. Tubercle 67:55–60
    [Google Scholar]
  4. Daniel T. M., Anderson P. A. 1977; The use of immunosorbents for the purification of mycobacterial antigens. Journal of Laboratory and Clinical Medicine 90:354–360
    [Google Scholar]
  5. Daniel T. M., Benjamin R. G., Debanne S. M., Ma Y., Balestrino E. A. 1985; ELISA of IgG antibody to M tuberculosis antigen 5 for serodiagnosis of tuberculosis. Indian Journal of Pediatrics 52:349–355
    [Google Scholar]
  6. Daniel T. M., Ferguson L. E. 1970; The purification and characterization of two proteins from culture filtrates of Mycobacterium tuberculosis, H37Ra strain. Infection and Immunity 1:164–168
    [Google Scholar]
  7. Krambovitis E. 1986; Detection of antibodies to Mycobacterium tuberculosis plasma membrane antigen by enzyme-linked immunosorbent assay. Journal of Medical Microbiology 21:257–264
    [Google Scholar]
  8. Krambovitis E., Harris M., Hughes D. T. D. 1986; Improved serodiagnosis of tuberculosis using two assay test. Journal of Clinical Pathology 39:779–785
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Nassau E., Parsons E. R., Johnson G. D. 1976; The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA). Tubercle 57:67–70
    [Google Scholar]
  11. Reggiardo Z., Vazquez E., Schnaper L. 1980; ELISA test for antibodies against mycobacterial glycolipids. Journal of Immunological Methods 34:55–60
    [Google Scholar]
  12. Stroebel A. B., Daniel T. M., Lau J. H. K., Leong J. C. Y., Richardson H. 1982; Serologic diagnosis of bone and joint tuberculosis by an enzyme-linked immunosorbent assay. Journal of Infectious Diseases 146:280–283
    [Google Scholar]
  13. Thongkrajai P., Chamnanvanakit C. 1986; An assessment of the enzyme-linked immunosorbent assay (ELISA) for diagnosis of pulmonary tuberculosis. Journal of the Medical Association of Thailand 69:324–329
    [Google Scholar]
  14. Vestal A. L. 1975; Procedures for the isolation and identification of mycobacteria. Department of Health, Education and Welfare, Center for Disease Control; Atlanta, GA:
    [Google Scholar]
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