Virulent Salmonella typhimurium, 2000 LD50, were injected intraperito-neally into inbred male A/J mice, genetically resistant to salmonella infection. Peritoneal exudate cells were harvested between 5 and 54 h after infection and examined by electronmicroscopy. Polymorphs were seen ingesting as well as digesting the pathogens as early as 5 h after infection. Macrophages were equally active in destroying the phagocytosed organisms throughout this period. In the meantime, the proliferation of salmonellae appeared to occur extracellularly in the peritoneal cavity as evidenced by their division.
BaronE. J.,
ProctorR. A.1984; Inefficient in vitro killing of virulent or nonvirulent Salmonella typhimurium by murine polymorphonuclear neutrophils. Canadian Journal of Microbiology 30:1264–1270
GuoY. N.,
HsuH. S.,
MumawV. R.,
NakonecznaI.1986a; Electronmicroscopy studies on the bactericidal action of inflammatory leukocytes in murine salmonellosis. Journal of Medical Microbiology 21:151–159
GuoY. N.,
HsuH. S.,
MumawV. R.,
NakonecznaI.1986b; Electronmicroscopy studies on the opsonic role of antiserum and the subsequent destruction of salmonellae within murine inflammatory leukocytes. Journal of Medical Microbiology 22:343–349
HsuH. S.,
MayoD. R.1973; Interactions between macrophages of guinea pigs and salmonellae. III. Bactericidal action and cytophilic antibodies of macrophages of infected guinea pigs. Infection and Immunity 8:165–172
HsuH. S.,
RadcliffeA. S.1968; Interactions between macrophages of guinea pigs and salmonellae. I. Fate of Salmonella typhimurium within macrophages of normal guinea pigs. Journal of Bacteriology 96:191–197
LinF. R.,
WangX. M.,
HsuH. S.,
MumawV. R.,
NakonecznaI.1987; Electron Microscopic studies on the location of bacterial proliferation in the liver in murine salmonellosis. British Journal of Experimental Pathology 68:539–550
LissnerC. R.,
SwansonR. N., O’ BrienA. D.1983; Genetic control of the innate resistance of mice to Salmonella typhimurium: expression of the Ity gene in peritoneal and splenic macrophages isolated in vitro. Journal of Immunology 131:3006–3013
MareckiN. M.,
HsuH. S.,
MayoD. R.1975; Cellular and humoral aspects of host resistance in murine salmonellosis. British Journal of Experimental Pathology 56:231–243
MayoD. R.,
HsuH. S.,
LimF.1977; Interactions between salmonellae and macrophages of guinea pigs. IV. Relationship between migration inhibition and antibacterial action of macrophages. Infection and Immunity 18:52–59
RhodesM. W.,
HsuH. S.1974; The effect of kanamycin on the fate of Salmonella enteritidis within cultured macrophages of guinea pigs. Journal of the Reticuloendothelial Society 15:1–12
SwansonR. N., O’ BrienA. D.1983; Genetic Control of the innate resistance of mice to Salmonella typhimurium: Ity gene is expressed in vivo by 24 hours after infection. Journal of Immunology 131:3014–3020
TakeuchiA.1967; Electron microscope studies on experimental salmonella infection. I. Penetration into the intestinal epithelium by Salmonella typhimurium. American Journal of Pathology 50:109–136
van DisselJ. T.,
LeijhP. C. J.,
van FurthR.1985; Differences in initial rate of intracellular killing of Salmonella typhimurium by resident peritoneal macrophages from various mouse strains. Journal of Immunology 134:3404–3410
van DisselJ. T.,
StikkelbroeckJ. J. M.,
SluiterW.,
LeijhP. C. J.,
van FurthR.1986; Differences in initial rate of intracellular killing of Salmonella typhimurium by granulocytes of salmonella-susceptible C57BL/10 mice and salmonella-resistant CBA mice. Journal of Immunology 136:1074–1080
WangX. M.,
LinF. R.,
HsuH. S.,
MumawV. R.,
NakonecznaI.1988; Electronmicroscopic studies on the location of salmonella proliferation in the murine spleen. Journal of Medical Microbiology 25:41–47