1887

Abstract

Summary

Six isolates of , representing various serotypes of the organism and possessing similar degrees of virulence in mice, were examined for their production of an extracellular toxic complex (ETC) . This compound is lethal for mice and produces extensive lung pathology in rats; it is composed of a surface carbohydrate antigen, lipopolysaccharide and protein. All six isolates produced the ETC. The LD50 values for the six ETC preparations ranged from 395 μg for strain 61g to 1750 μg for strain 90ee. Only two of the six ETC preparations contained ketodeoxyoctonate detectable by the methods used, and these two were the most toxic. Rabbit antiserum to the ETC of a serotype D strain could significantly protect mice only against serotype D strains. Examination of the various phases of growth of showed that there was extracellular release of the ETC beginning in the early logarithmic phase and continuing through the late stationary phase. The presence of the ETC in the supernatant fluids was due to release of this material rather than to cell lysis. In addition, at least one strain of was shown to produce an alginic acid-like compound.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-30-1-17
1989-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/30/1/medmicro-30-1-17.html?itemId=/content/journal/jmm/10.1099/00222615-30-1-17&mimeType=html&fmt=ahah

References

  1. Anwar H., Brown M. R. W., Cozens R. M., Lambert P. A. 1983a; Isolation and characterization of the outer and cytoplasmic membranes of Pseudomonas cepacia. Journal of General Microbiology 129:499–507
    [Google Scholar]
  2. Anwar H., Brown M. R. W., Lambert P. A. 1983b; Effect of nutrient depletion on sensitivity of Pseudomonas cepacia to phagocytosis and serum bactericidal activity at different temperatures. Journal of General Microbiology 129:2031–2027
    [Google Scholar]
  3. Blumenkrantz N., Asboe-Hansen G. 1973; New method for quantitative determination of uronic acids. Analytical Biochemistry 54:484–489
    [Google Scholar]
  4. Carlson D. M., Matthews L. W. 1966; Polyuronic acids produced by Pseudomonas aeruginosa. Biochemistry 5:2817–2822
    [Google Scholar]
  5. Chedid L., Skarnes R. C., Parant M. 1963; Characterization of aCr51-labeled endotoxin and its identification in plasma and urine after parenteral administration. Journal of Experimental Medicine 117:561–571
    [Google Scholar]
  6. Corey M., Allison L., Prober C., Levison H. 1984; Sputumbacteriology in patients with cystic fibrosis in a Toronto hospital during 1970–1981. Journal of Infectious Diseases 149:283
    [Google Scholar]
  7. Evans L. R., Linker A. 1973; Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa. Journal of Bacteriology 116:915–924
    [Google Scholar]
  8. Gankema H., Wensink J., Guinee P. A. M., Jansen W. H., Witholt B. 1980; Some characteristics of the outer membrane materialreleased by growing enterotoxigenic Escherichia coli.. Infection and Immunity 29:704–713
    [Google Scholar]
  9. Goldmann D. A., Klinger J. D. 1986; Pseudomonas cepacia: biology, mechanisms of virulence and epidemiology. Journal of Pediatrics 108:806–812
    [Google Scholar]
  10. Isles A. 1984; Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. Journal of Pediatrics 104:206–210
    [Google Scholar]
  11. Jonsson V. 1970; Proposal of a new species Pseudomonas kingii. International Journal of Systematic Bacteriology 20:255–257
    [Google Scholar]
  12. Linker A., Jones R. S. 1966; A new polysaccharide resemblingalginic acid isolated from Pseudomonads. Journal of Biological Chemistry 241:3845–3851
    [Google Scholar]
  13. Mackowiak P. A. 1984; Relationship between growth temperature and shedding of lipopolysaccharides by gram-negative bacilli. European Journal of Clinical Microbiology 3:406–410
    [Google Scholar]
  14. Manniello J. M., Heymann H., Adair F. W. 1979; Isolation of a typical lipopolysaccharides from purified cell walls of Pseudomonas cepacia. Journal of General Microbiology 112:397–400
    [Google Scholar]
  15. McComb E. A., McCready R. M. 1957; Determination of acetyl inpectin and in acetylated carbohydrate polymers. Hydroxamic acid reaction. Analytical Chemistry 29:819–821
    [Google Scholar]
  16. McKevitt A. I., Retzer M. D., Woods D. E. 1987; Development and use of a serotyping scheme for Pseudomonas cepacia. Serodiagnosis and Immunotherapy 1:177–184
    [Google Scholar]
  17. Osborn M. J. 1963; Studies on the gram-negative cell wall. I.Evidence for the role of 2-keto-3-deoxyoctonate in thelipopolysaccharide of Salmonella typhimurium. Proceedings of the National Academy of Science of the USA 50:499–506
    [Google Scholar]
  18. Reed L. J., Muench H. 1938; A simple method of estimating fifty-percent end points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  19. Rietschel E. T. 1988; Bacterial endotoxins: properties andstructure of biologically active domains. In Schrinner E. etal. (eds) Surface structures of micro organisms and their interactions with the mammalian host VCH; New York: pp 1–38
    [Google Scholar]
  20. Rothfield L., Pearlman-Kothencz M. 1969; Synthesis and assembly of bacterial membrane components. A lipopoly saccharide-phospholipid-protein complex excreted by living bacteria. Journal of Molecular Biology 44:477–492
    [Google Scholar]
  21. Russell R. R. B. 1976; Free endotoxin—a review. Microbios Letters 2:125–135
    [Google Scholar]
  22. Staub A. M. 1967; Preparation of antigens and antibodies. In Williams C. A., Chase M. W. (eds) Methods in immunology and immunochemistry vol I Academic Press; New York: pp 28–34
    [Google Scholar]
  23. Straus D. C. 1987; Production of an extracellular toxic complex by various strains of Klebsiella pneumoniae. Infection and Immunity 55:44–48
    [Google Scholar]
  24. Straus D. C., Woods D. E., Lonon M. K., Garner C. W. 1988; The importance of extra cellular antigens in Pseudomonas cepacia infections. Journal of Medical Microbiology 26:269–280
    [Google Scholar]
  25. Sullivan J. D., Valois F. W., Watson S. W. 1976; Endotoxins: The Limulus amebocyte system. In Bemheimer A. W. (ed) Mechanisms in bacterial toxinology John Wiley and Sons; New York: p 217
    [Google Scholar]
  26. Thomassen M. J., Demko C. A., Doershuk C. F., Stern R. C., KlingerJ D. 1986; Pseudomonas cepacia:decrease in colonization inpatients with cystic fibrosis. American Review of Respiratory Diseases 134:669–671
    [Google Scholar]
  27. Thomassen M. J., Demko C. A., Klinger J. D., Stern R. C. 1985; Pseudomonas cepacia colonization among patients with cystic fibrosis. American Review of Respiratory Diseases 131:791–796
    [Google Scholar]
  28. Uehara K., Fujimoto S., Taniguchi K., Nakai K. 1974; Studies on violet–colored acid phosphatase of sweet potato. II. Enzymatic properties and amino acid composition. Journal of Biochemistry 75:639–649
    [Google Scholar]
  29. Vadas L., Prihar H. S., Pugashetti B. K., Feingold D. S. 1981; A gas chromatographic method for the quantitative determination of hexuronic acids in alginic acid. Analytical Biochemistry 114:294–298
    [Google Scholar]
  30. Young L. S. 1972; Human immunity to Pseudomonas aeruginosaII.Relationship between heat-stable opsonins and type-specific lipopoly saccharides. Journal of Infectious Diseases 126:277–287
    [Google Scholar]
  31. Young L. S., Armstrong D. 1972; Human immunity to Pseudomonasaeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors. Journal of Infectious Diseases 126:257–276
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-30-1-17
Loading
/content/journal/jmm/10.1099/00222615-30-1-17
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error