1887

Journal of Medical Microbiology logo

Volume 3, Issue 4

An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus Free

Abstract

SUMMARY

Fowls were infected with avian-IBV and, subsequently, their tracheas were sectioned and examined in the electron microscope. The lining epithelial cells showed extensive loss of cilia, degeneration of microvilli, swelling of mitochondria, increased amount of endoplasmic reticulum and of other intracellular membranes, and the presence of electron-dense areas in the cytoplasm. Virus particles were first observed about 12 hr after infection within the electrondense areas and within spaces between the membranes of the endoplasmic reticulum. By 48–72 hr they were considerably more numerous and were present mainly within cisternae and vesicles formed from the membranes of the endoplasmic reticulum. There was little evidence of budding. The mature virus particle had a mean diameter of 81±1 nm and possessed both inner and outer electron-dense coats or shells separated by an electron-transparent layer. Virus particles grown in the embryonated hen egg and harvested from the allantoic fluid had a similar size and structure.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1970
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-3-4-643
1970-11-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/3/4/medmicro-3-4-643.html?itemId=/content/journal/jmm/10.1099/00222615-3-4-643&mimeType=html&fmt=ahah

References

  1. Almeida June D., Tyrrell D. A. J. 1967 J. Gen. Virol 1:175
     [Google Scholar]
  2. Becker W. B., McIntosh K., Dees June N., Chanock R. M. 1967 J. Virol 1:1019
     [Google Scholar]
  3. Berry D. M., Cruikshank J. G., Chu H. P., Wells R. J. H. 1964 Virology 23:403
     [Google Scholar]
  4. Chu H. P. 1964; Newcastle disease virus; an evolving pathogen. Proc. Int. Symp., Univ. Wisconsin, 1963 ed. by Hanson R. P. Madison, Wisconsin, USA:
     [Google Scholar]
  5. Dalton A. J. 1955 Anat. Rec 121:281
     [Google Scholar]
  6. Domermuth C. H., Edwards O. F. 1957 J. Infect. Dis 100:74
     [Google Scholar]
  7. Echlin P. 1964 Arch. Mikrobiol 49:267
     [Google Scholar]
  8. Lascano E. F., Berria Maria I., Barrera Oro J. G. 1969 J. Virol 4:271
     [Google Scholar]
  9. Lecatsas G. 1968 Onderstepoort J. Vet. Res 35:139
     [Google Scholar]
  10. Mcintosh K., Dees June N., Becker W. B., Kapikian A. Z., Chanock R. M. 1967 Proc. Natn. Acad. Sci., USA 57:933
     [Google Scholar]
  11. Murphy F. A., Coleman P. H., Harrison Alyne K., Gary G. W. Jr 1968 Virology 37:28
     [Google Scholar]
  12. Nazerian K., Cunningham C. H. 1968 J. Gen. Viral 3:469
     [Google Scholar]
  13. Reagan R. L., Brueckner A. L. 1952 Amer. J. Vet. Res 13:417
     [Google Scholar]
  14. Reagan R. L., Hauser J. E., Lillie M. G., Craigde A. H. Jr 1948 Cornell Vet 38:190
     [Google Scholar]
  15. Richardson K. C., Jarett L., Finke E. H. 1960 Stain Technol 35:313
     [Google Scholar]
  16. Tyrrell D. A. J., Bynoe M. L. 1965 Br. Med J 1:1467
     [Google Scholar]
  17. Tyrrell D. A. J., Bynoe M. L., Hoorn B. 1968 Ibid 1:606
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-3-4-643
Loading
An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus
J. Med. Microbiol. 3, 643 (1970); https://doi.org/10.1099/00222615-3-4-643
/content/journal/jmm/10.1099/00222615-3-4-643
/content/journal/jmm/10.1099/00222615-3-4-643
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error