The extent of placental localisation of after experimental infection of pregnant ewes has been examined and a nutritional basis for the localisation has been investigated. After infection, was found only in the uterine contents; the placenta and chorion were most consistently infected. In advanced infection, quantitative measurement revealed that the placenta, allantoic fluid and chorion together contained 80–90 per cent. of the total organisms present.

Erythritol, the factor responsible for the localisation of spp. in the bovine and ovine placenta, failed to stimulate the growth of in a range of media. The rate of growth of in extracts of foetal cotyledon was greater than that observed when the organism was grown in extracts of most other tissues; this finding supports the hypothesis that the tissue predilection is dependent on local nutrients.


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