Preparations of colicines A, B, D, E, E and K were exposed in separate experiments to chloroform, heat, filtration, and ultraviolet light and the effects were examined quantitatively.

Chloroform at a concentration of 5 per cent. (v/v) sterilised preparations of each colicine within 5 min. and there was an acceptably low degree of inactivation. Exposure for longer periods was associated with progressive inactivation of B colicines and colicine K.

Millipore filtration and ultraviolet irradiation are slightly more effective but technically more exacting methods applicable to large and small samples respectively. Seitz filtration removed all detectable colicine activity.

Heating is not recommended as its effects on colicine activity are difficult to predict. The criteria of thermostability are discussed.


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