1887

Journal of Medical Microbiology logo

Volume 3, Issue 4

Sterilisation Of Colicine Preparations Free

Abstract

SUMMARY

Preparations of colicines A, B, D, E, E and K were exposed in separate experiments to chloroform, heat, filtration, and ultraviolet light and the effects were examined quantitatively.

Chloroform at a concentration of 5 per cent. (v/v) sterilised preparations of each colicine within 5 min. and there was an acceptably low degree of inactivation. Exposure for longer periods was associated with progressive inactivation of B colicines and colicine K.

Millipore filtration and ultraviolet irradiation are slightly more effective but technically more exacting methods applicable to large and small samples respectively. Seitz filtration removed all detectable colicine activity.

Heating is not recommended as its effects on colicine activity are difficult to predict. The criteria of thermostability are discussed.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1970
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-3-4-585
1970-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/3/4/medmicro-3-4-585.html?itemId=/content/journal/jmm/10.1099/00222615-3-4-585&mimeType=html&fmt=ahah

References

  1. Barry G. T., Everhart D. L., Graham M. 1963 Nature, Lond 198:211
     [Google Scholar]
  2. Clarke C. H., Macphee D. G. 1965 Experientia 21:18
     [Google Scholar]
  3. Fastier L. B. 1949 J. Immun 62:399
     [Google Scholar]
  4. Fredericq P. 1957 A. Rev. Microbiol 11:7
     [Google Scholar]
  5. Fredericq P. 1963 Ergebn. Mikrobiol. ImmunForsch. exp. Ther 37:114
     [Google Scholar]
  6. Fredericq P., Betz-Barreau M. 1948 C.r. Séanc. Soc. Biol 142:1078
     [Google Scholar]
  7. Hamon Y., Péron Yvonne. 1959 Annls Inst. Pasteur, Paris 97:518
     [Google Scholar]
  8. Hamon Y., Péron Yvonne. 1961 Ibid 100:818
     [Google Scholar]
  9. Hamon Y., Péron Yvonne. 1963 Ibid 104:127
     [Google Scholar]
  10. Hamon Y., Péron Yvonne. 1964 Ibid 107:44
     [Google Scholar]
  11. Hamon Y., Péron Yvonne. 1966 Ibid 110:389
     [Google Scholar]
  12. Hauduroy P., Papavassiliou J. 1962 Ibid 102:644
     [Google Scholar]
  13. Heatley N. G., Florey H. W. 1946 Br. J. Exp. Path 27:378
     [Google Scholar]
  14. Keene J. H. 1966 Canad. J. Microbiol 12:425
     [Google Scholar]
  15. Kellenberger G., Kellenberger E. 1956 Schweiz. Z. allg. Path. Bakt 19:582
     [Google Scholar]
  16. Matsushita H., Fox M. S., Goebel W. F. 1960 J. Exp. Med 112:1055
     [Google Scholar]
  17. Mukaj F. H. 1960 J. Gen. Microbiol 23:539
     [Google Scholar]
  18. Nomura M. 1967 A. Rev. Microbiol 21:257
     [Google Scholar]
  19. Papavassiliou J. 1961 Nature, Lond 190:110
     [Google Scholar]
  20. Reeves P. R. 1963 Austral. J. Exp. Biol. Med. Sci 41:163
     [Google Scholar]
  21. Richardson H., Emslie-Smith A. H., Senior B. W. 1968 Appl. Microbiol 16:1468
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-3-4-585
Loading
Sterilisation Of Colicine Preparations
J. Med. Microbiol. 3, 585 (1970); https://doi.org/10.1099/00222615-3-4-585
/content/journal/jmm/10.1099/00222615-3-4-585
/content/journal/jmm/10.1099/00222615-3-4-585
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error