1887

Abstract

Summary

Preparations of colicines A, B, D, E, E and K were exposed in separate experiments to chloroform, heat, filtration, and ultraviolet light and the effects were examined quantitatively.

Chloroform at a concentration of 5 per cent. (v/v) sterilised preparations of each colicine within 5 min. and there was an acceptably low degree of inactivation. Exposure for longer periods was associated with progressive inactivation of B colicines and colicine K.

Millipore filtration and ultraviolet irradiation are slightly more effective but technically more exacting methods applicable to large and small samples respectively. Seitz filtration removed all detectable colicine activity.

Heating is not recommended as its effects on colicine activity are difficult to predict. The criteria of thermostability are discussed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-3-4-585
1970-11-01
2020-01-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-3-4-585
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error