Colonies of T-mycoplasmas isolated from a monkey, a dog, a bull and man lysed erythrocytes suspended in an agar overlay. Haemolysis was of the α' (alpha-prime) type, and was usually detectable only with the low power of the microscope. As compared with , T-mycoplasma haemolysis occurred inconsistently in repeated tests. Crowding of colonies inhibited haemolysis. The addition of catalase also inhibited haemolysis indicating that the T-mycoplasma haemolysin is a peroxide. This haemolysin is probably produced in only small amounts. Protection of erythrocytes against lysis in the close proximity of the colonies of T-mycoplasmas and was also observed. This phenomenon occurred as frequently as haemolysis, and may have contributed to the difficulty experienced in detecting weak haemolysis. It too was inhibited by crowding of colonies. The protective effect was not due to catalase production, and it is suggested that a peroxidase might be responsible.


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