%0 Journal Article %A Campbell, J. G. %A Woode, G. N. %T Demonstration Of A Herpes-Type Virus In Short-Term Cultured Blood Lymphocytes Associated With Marek’S Disease %D 1970 %J Journal of Medical Microbiology, %V 3 %N 3 %P 463-473 %@ 1473-5644 %R https://doi.org/10.1099/00222615-3-3-463 %I Microbiology Society, %X SUMMARY Electron microscopy of short-term cultured blood lymphocytes from spontaneous cases of “classical” (neural) Marek’s disease and some clinically normal chickens from the same flock showed a herpes-type intranuclear virus in a high proportion of transformed cells. In a few instances virus particles were closely associated with intranuclear filaments, which were thought to represent the product of aberrant viral replication. Some non-transformed cells showed granule-lamellar arrays in the cytoplasm, thought possibly to represent the inner structure of the filaments. The addition of cultured lymphocytes to healthy chick kidney monolayers induced a typical transmissible cytopathic effect associated with nuclear inclusions. Similar results were obtained using fresh lymphocytes, or tumour cells, from cases of the “acute” disease. A spontaneous cytopathic effect often developed in monolayers prepared from clinically normal chickens in a flock in which the disease was endemic. Indirect immunofluorescence tests with either classical or acute Marek’s disease antisera indicated the presence of viral antigen in a high proportion of transformed lymphocytes and in the kidney cells in the region of the cytopathic effect. A herpes-type virus was also demonstrated in cultured transformed blood lymphocytes in cases of acute Marek’s disease with gonadal tumours. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-3-3-463